Ma Fei-Yue, Zhang Xiu-Mei, Li Ya, Zhang Ming, Tu Xing-Hao, Du Li-Qing
South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), Zhanjiang, China.
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, China.
Front Nutr. 2022 Aug 15;9:970019. doi: 10.3389/fnut.2022.970019. eCollection 2022.
Miracle berry is well-known for its ability to convert sour foods to sweet. In this study, the secondary metabolites of miracle berry leaves (MBL) were identified by UPLC-DAD-MS, and its antiangiogenesis and anticancer activities were evaluated by using a zebrafish model and the MCF-7 xenograft mouse model, respectively. The result showed that 18 phenolic compounds were identified in MBL extract, and dominated by the derivatives of quercetin and myricetin. The MBL extract showed low toxicity and high antiangiogenesis activity, it significantly inhibited the subintestinal vein vessels development in zebrafish at very low concentration. Furthermore, the MBL extract could promote the apoptosis of tumor cells and significantly inhibit the growth of MCF-7 xenograft tumor. In addition, the analysis of metabolites revealed that the MBL extract inhibited tumor growth by activating the metabolic pathways of unsaturated fatty acids and purines. Overall, this study suggests that MBL extract can be used as a natural anticancer adjuvant in the fields of functional foods.
奇迹果以其将酸味食物转化为甜味的能力而闻名。在本研究中，通过超高效液相色谱-二极管阵列-质谱联用仪（UPLC-DAD-MS）鉴定了奇迹果叶（MBL）的次生代谢产物，并分别使用斑马鱼模型和MCF-7异种移植小鼠模型评估了其抗血管生成和抗癌活性。结果表明，在MBL提取物中鉴定出18种酚类化合物，以槲皮素和杨梅素的衍生物为主。MBL提取物显示出低毒性和高抗血管生成活性，在极低浓度下就能显著抑制斑马鱼的肠下静脉血管发育。此外，MBL提取物可促进肿瘤细胞凋亡并显著抑制MCF-7异种移植肿瘤的生长。另外，代谢物分析表明，MBL提取物通过激活不饱和脂肪酸和嘌呤的代谢途径来抑制肿瘤生长。总体而言，本研究表明MBL提取物可在功能性食品领域用作天然抗癌佐剂。
