多重短串联重复序列分析永生化肝星状细胞系 Col-GFP HSC。
Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC.
机构信息
Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, Aachen, Germany.
Department of Medicine, University of California San Diego, La Jolla, CA, United States of America.
出版信息
PLoS One. 2022 Sep 6;17(9):e0274219. doi: 10.1371/journal.pone.0274219. eCollection 2022.
Misidentification, cross-contamination and genetic drift of continuous animal cell lines are persistent problems in biomedical research, leading to erroneous results and inconsistent or invalidated studies. The establishment of immortalized hepatic stellate cell line Col-GFP HSC was reported in PLoS One in the year 2013. In the present study a multi loci short tandem repeat signature for this cell line was established that allows for unique cell line authentication.
在生物医学研究中，连续动物细胞系的错误鉴定、交叉污染和遗传漂变是持续存在的问题，导致错误的结果和不一致或无效的研究。2013 年，《公共科学图书馆·综合》杂志报道了建立永生化肝星状细胞系 Col-GFP HSC。本研究建立了该细胞系的多位点短串联重复序列特征，可实现独特的细胞系鉴定。
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