将PETase酶固定在磁性氧化铁纳米颗粒上用于分解微塑料聚对苯二甲酸乙二酯
Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET.
作者信息
Schwaminger Sebastian P, Fehn Stefan, Steegmüller Tobias, Rauwolf Stefan, Löwe Hannes, Pflüger-Grau Katharina, Berensmeier Sonja
机构信息
Department of Mechanical Engineering, Bioseparation Engineering Group, Technical University of Munich Garching Germany
Department of Mechanical Engineering, Systems Biotechnology, Technical University of Munich Garching Germany.
出版信息
Nanoscale Adv. 2021 Jun 14;3(15):4395-4399. doi: 10.1039/d1na00243k. eCollection 2021 Jul 27.
Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.
聚对苯二甲酸乙二酯（PET）造成了大量微塑料对环境的污染。基于其高亲和力，PET降解酶PETase可通过His标签固定在超顺磁性氧化铁纳米颗粒上。His标签提高了酶的稳定性，并允许通过磁分离进行回收。多个循环步骤是可行的，并且微塑料颗粒可以根据PET的结晶度进行分解。PET的分离或分解为从水中去除微塑料提供了一种可持续的方法。
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