Instituto de Biodiversidade e Florestas (IBEF), Universidade Federal do Oeste do Pará (UFOPA), Santarem 68040-255, Brazil.
Programa de Pós-Graduação em Sociedade, Natureza e Desenvolvimento (PPGSND), Universidade Federal do Oeste do Pará (UFOPA), Santarem 68040-255, Brazil.
Molecules. 2022 Sep 8;27(18):5837. doi: 10.3390/molecules27185837.
and are species native to the Amazonian, traded by extractivists to obtain coumarin. We aimed to analyze the presence of coumarin in the ethanolic extracts of leaves, branches and fruits of and and to evaluate the antimicrobial activity of these extracts against phytopathogenic fungi and bacteria of clinical interest. Chemical analyses were performed by thin layer chromatography (TLC) and by gas chromatography coupled to mass spectrometry (GC-MS). For the antifungal assays, the fungi used were , , two isolates of spp. and , and the antibacterial assay was performed using the minimum inhibitory concentration (MIC) test with , , and bacteria. In seed extracts and in husks, endocarps, and seeds, we identified 1,2-benzopyrone. endocarp extracts and seeds provided the greatest decrease in mycelial growth of the evaluated phytopathogens, showing promise as an alternative control. The husk and endocarp extracts of both species had a weak effect on . This research is the first to compare the different parts of species of the genus and to evaluate the use of husks and endocarps of fruits to obtain coumarin. Chemical analyses used to quantify the compounds existing in the extracts, and tests with phytopathogens in vitro and in vivo are currently being carried out.
和 是原产于亚马逊地区的物种,被采集者交易以获取香豆素。我们旨在分析 和 的叶、枝和果的乙醇提取物中香豆素的存在,并评估这些提取物对具有临床意义的植物病原真菌和细菌的抗菌活性。化学分析通过薄层色谱 (TLC) 和气相色谱-质谱联用 (GC-MS) 进行。对于抗真菌试验,使用的真菌为 、 、两种 spp. 的分离株和 ,而抗菌试验则使用最小抑菌浓度 (MIC) 试验,用 、 、 和 细菌进行。在 种子提取物以及 果壳、中果皮和种子中,我们鉴定出了 1,2-苯并吡喃酮。 中果皮提取物和 种子对评估的植物病原菌的菌丝生长有最大的抑制作用,显示出作为替代控制的潜力。这两个物种的果壳和中果皮提取物对 的影响较弱。这项研究首次比较了该属不同物种的不同部位,并评估了利用 和 的果壳获取香豆素。目前正在进行用于定量提取物中存在化合物的化学分析,以及在体外和体内进行的植物病原菌试验。