Alasmar Reem Moath, Varadharajan Kavitha, Shanmugakonar Muralitharan, Al-Naemi Hamda A
Laboratory Animal Research Centre, Qatar University, Doha, Qatar.
Department of Biological and Environmental Sciences, Qatar University, Doha, Qatar.
Mol Nutr Food Res. 2023 Dec;67(24):e2200322. doi: 10.1002/mnfr.202200322. Epub 2022 Oct 19.
The composition of the gut microbiota is influenced by the dietary nutrient. Sugar has been linked with many metabolic health disorders such as heart disease, metabolic syndrome, and immune disorders. Long-term consumption of sugar influences the landscape of gut microbiota by altering the gut microbial population called dysbiosis. This study aims to evaluate the impact of long-term consumption of high sugar diet (HSD) on the diversity of gut microbiota.
CD1 mice are given high concentration of sugar for 15 weeks followed by a recovery period of 10 weeks. Real-time polymerase chain reaction and 16S rRNA next-generation sequencing methods employ to identify microbiome diversity. The results show that Firmicutes and Bacteroidetes are the predominant phyla in control, cecum, and fecal samples. Firmicutes population are gradually increased in treated samples even after the recovery period, whereas Bacteroidetes abundance slightly reduces throughout the study.
The present study shows that the impact of long period of high sugar diet consumption alters the diversity of normal gut flora which can be restored after 10 weeks of sugar withdrawal. This indicates that the intervention of healthy and nutritious diet influences gut microbes and this can be beneficial in reducing the implication of early life metabolic disorders such as obesity.
肠道微生物群的组成受饮食营养的影响。糖已与许多代谢性健康疾病相关联，如心脏病、代谢综合征和免疫紊乱。长期食用糖会通过改变称为生态失调的肠道微生物种群来影响肠道微生物群的格局。本研究旨在评估长期食用高糖饮食（HSD）对肠道微生物群多样性的影响。
给CD1小鼠喂食高浓度糖15周，随后有10周的恢复期。采用实时聚合酶链反应和16S rRNA下一代测序方法来鉴定微生物群多样性。结果表明，厚壁菌门和拟杆菌门是对照、盲肠和粪便样本中的主要门类。即使在恢复期后，处理过的样本中厚壁菌门的数量仍逐渐增加，而在整个研究过程中拟杆菌门的丰度略有降低。
本研究表明，长期食用高糖饮食的影响会改变正常肠道菌群的多样性，在停止食用糖10周后这种多样性可以恢复。这表明健康营养饮食的干预会影响肠道微生物，这可能有助于减少肥胖等早期生活代谢紊乱的影响。
