诱导减毒鮣诺卡氏菌及其作为活疫苗试验对鱼类诺卡氏菌病的应用。
Induction of attenuated Nocardia seriolae and their use as live vaccine trials against fish nocardiosis.
机构信息
Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, Guangdong, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Guangdong Ocean University, Zhanjiang, Guangdong, China.
Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, Guangdong, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Guangdong Ocean University, Zhanjiang, Guangdong, China.
出版信息
Fish Shellfish Immunol. 2022 Dec;131:10-20. doi: 10.1016/j.fsi.2022.09.053. Epub 2022 Sep 24.
Nocardia seriolae, a Gram-positive facultative intercellular pathogen, has been identified as the causative agent of fish nocardiosis, causing substantial mortality and morbidity of a wide range of fish species. Looking into that fact, the effective vaccine against this pathogen is urgently needed to control the significant losses in aquaculture practices. In order to induct attenuated strains for developing the potential live vaccines, the mutagenic N. seriolae strain S-250 and U-20 were obtained from wild-type strain ZJ0503 through continuous passaging and ultraviolet (UV) irradiation, respectively. Additionally, the biological characteristic, virulence, stability, mediating immune response and supplying protective efficacy to hybrid snakehead of the S-250 and U-20 strains were determined in the present study. The results showed that U-20 strain displayed dramatic changes in morphological characteristic and significant decreased in the virulence to hybrid snakehead, while that of S-250 strain had no obvious different in comparison to ZJ0503 strain. When hybrid snakehead were intraperitoneally injected with ZJ0503, S-250 and U-20 strains at their respective sub-clinical dosage, the non-specific immunity parameters (serum LYZ, POD, ACP, AKP and SOD activities), specific antibody (IgM) titers production and immune-related genes (CC1, CC2, IL-1β, IL-8, TNFα, IFNγ, MHCIα, MHCIIα, CD4, CD8α, TCRα and TCRβ) expression were up-regulated, indicating that they were able to trigger humoral and cell-mediated immune responses. Furthermore, the protective efficacy in hybrid snakehead after vaccination with ZJ0503, S-250 and U-20 strains, in terms of relative percentage survival (RPS), were 28.85%, 56.89% and 89.65% respectively. Taken together, two attenuated N. seriolae strains S-250 and U-20 were obtained successfully and they could elicit strong immune response and supply protective efficacy to hybrid snakehead against N. seriolae, which suggested that these two attenuated strains were the potential candidates for live vaccine development to control fish nocardiosis in aquaculture.
杀鲑气单胞菌是一种革兰氏阳性兼性细胞内病原体，已被确定为鱼类诺卡氏菌病的病原体，导致多种鱼类的大量死亡和发病。鉴于这一事实，迫切需要开发针对这种病原体的有效疫苗，以控制水产养殖实践中的重大损失。为了诱导减毒株以开发潜在的活疫苗，通过连续传代和紫外线（UV）照射，分别从野生型菌株 ZJ0503 中获得了突变的杀鲑气单胞菌 S-250 和 U-20 菌株。此外，本研究还确定了 S-250 和 U-20 菌株的生物学特性、毒力、稳定性、介导的免疫反应和对杂交乌鳢的保护效力。结果表明，U-20 菌株的形态特征发生了明显变化，对杂交乌鳢的毒力显著降低，而 S-250 菌株与 ZJ0503 菌株相比没有明显变化。当杂交乌鳢分别以亚临床剂量腹腔注射 ZJ0503、S-250 和 U-20 菌株时，非特异性免疫参数（血清 LYZ、POD、ACP、AKP 和 SOD 活性）、特异性抗体（IgM）滴度产生和免疫相关基因（CC1、CC2、IL-1β、IL-8、TNFα、IFNγ、MHCIα、MHCIIα、CD4、CD8α、TCRα和 TCRβ）表达上调，表明它们能够引发体液和细胞介导的免疫反应。此外，用 ZJ0503、S-250 和 U-20 菌株接种后的杂交乌鳢的保护效力（RPS）分别为 28.85%、56.89%和 89.65%。综上所述，成功获得了两种减毒杀鲑气单胞菌 S-250 和 U-20 菌株，它们能够诱导杂交乌鳢产生强烈的免疫反应并提供针对杀鲑气单胞菌的保护效力，这表明这两种减毒菌株是开发控制水产养殖中鱼类诺卡氏菌病的活疫苗的潜在候选者。
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