大肠杆菌喹啉酸生物合成中中间体的证据。
Evidence for an intermediate in quinolinate biosynthesis in Escherichia coli.
作者信息
Wicks F D, Sakakibara S, Gholson R K
出版信息
J Bacteriol. 1978 Oct;136(1):136-41. doi: 10.1128/jb.136.1.136-141.1978.
Abstract
Evidence for the formation of an unstable intermediate in the synthesis of quinolinate from aspartate and dihydroxyacetone phosphate by Escherichia coli was obtained using toluenized cells of nadA and nadB mutants of this organism and partially purified A and B proteins in dialysis and membrane cone experiments. The results of these experiments indicate that the nadB gene product forms an unstable compound from aspartate in the presence of flavine adenine dinucleotide, and that this compound is then condensed with dihydroxyacetone phosphate to form quinolinate in a reaction catalyzed by the nadA gene product.
摘要
通过使用该生物体的nadA和nadB突变体的甲苯处理细胞以及透析和膜锥实验中部分纯化的A和B蛋白，获得了大肠杆菌由天冬氨酸和磷酸二羟丙酮合成喹啉酸过程中形成不稳定中间体的证据。这些实验结果表明，nadB基因产物在黄素腺嘌呤二核苷酸存在下由天冬氨酸形成一种不稳定化合物，然后该化合物在nadA基因产物催化的反应中与磷酸二羟丙酮缩合形成喹啉酸。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ENZYMATIC CONVERSION OF QUINOLINATE TO NICOTINIC ACID MONONUCLEOTIDE IN MAMMALIAN LIVER.哺乳动物肝脏中喹啉酸向烟酰胺单核苷酸的酶促转化
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Quinolinic acid: a precursor to nicotinamide adenine dinucleotide in Escherichia coli.喹啉酸：大肠杆菌中烟酰胺腺嘌呤二核苷酸的前体。
Biochem Biophys Res Commun. 1963 Jul 18;12:92-7. doi: 10.1016/0006-291x(63)90241-9.
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Pyrimidine biosynthesis in Escherichia coli.大肠杆菌中的嘧啶生物合成
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The conversion of N-formyl-L-aspartate into nicotinic acid by extracts of Clostridium butylicum.丁酸梭菌提取物将N-甲酰-L-天冬氨酸转化为烟酸。
Eur J Biochem. 1969 Sep;10(2):318-23. doi: 10.1111/j.1432-1033.1969.tb00692.x.
6
Effects of toluene on Escherichia coli.甲苯对大肠杆菌的影响。
J Bacteriol. 1965 Nov;90(5):1420-5. doi: 10.1128/jb.90.5.1420-1425.1965.
7
Current linkage map of Escherichia coli.大肠杆菌当前的连锁图谱。
Bacteriol Rev. 1970 Jun;34(2):155-75. doi: 10.1128/br.34.2.155-175.1970.
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Biosynthesis of quinolinic acid in a cell-free system.无细胞体系中喹啉酸的生物合成。
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