da Silva Nunes Paola Caroline, Mazzarella Rosane, da Silveira Juliano Coelho, Dellova Deise Carla Almeida Leite
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, 13635-900, Brazil.
Sci Rep. 2022 Sep 30;12(1):16439. doi: 10.1038/s41598-022-20523-x.
Adipose tissue is a metabolic and endocrine organ, and its adipocytes can synthesize and secrete extracellular vesicles (EVs), thus allowing intercellular communication. EVs are nanoparticles that transport lipids, proteins, metabolites, and nucleic acids (mRNA and microRNAs). MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression. miR-132, miR-26b, and miR-155 are associated with obesity, lipid metabolism and adipogenesis. The aim of this study was to evaluate the enriched EVs fraction containing miRNAs (miR-132, miR-26b, and miR-155) in serum from obese female dogs. Thirty-two neutered females in good general condition were recruited, including 21 obese and 11 healthy controls. The initial evaluation of the females included a general physical examination and laboratory tests. Small EVs (sEVs) were isolated from whole blood by serial centrifugation and ultracentrifugation, and nanoparticle analysis was used to determine the size and concentration of serum sEVs. miRNAs were extracted from sEVs enriched fraction and analyzed by real-time polymerase chain reaction. Obese female dogs with hypertriglyceridemia showed an increase in the sEVs concentration and in the expression of miR-132 and miR-26b in sEVs enriched fraction. No changes were observed in the group of obese female dogs with normal serum biochemical profile and in relation to miR-155 expression. These results suggest that obese female dogs with hypertriglyceridemia may present alterations in sEVs and in the expression of miRNAs related to lipid metabolism and adipogenesis.
脂肪组织是一个代谢和内分泌器官，其脂肪细胞能够合成并分泌细胞外囊泡（EVs），从而实现细胞间通讯。细胞外囊泡是运输脂质、蛋白质、代谢物和核酸（mRNA和微小RNA）的纳米颗粒。微小RNA（miRNAs）是调节基因表达的小的非编码RNA。miR - 132、miR - 26b和miR - 155与肥胖、脂质代谢和脂肪生成有关。本研究的目的是评估肥胖雌性犬血清中富含miRNAs（miR - 132、miR - 26b和miR - 155）的细胞外囊泡部分。招募了32只身体状况良好的绝育雌性犬，其中包括21只肥胖犬和11只健康对照犬。对这些雌性犬的初步评估包括全身体格检查和实验室检查。通过连续离心和超速离心从全血中分离出小细胞外囊泡（sEVs），并使用纳米颗粒分析来确定血清sEVs的大小和浓度。从富含sEVs的部分提取miRNAs，并通过实时聚合酶链反应进行分析。患有高甘油三酯血症的肥胖雌性犬的sEVs浓度以及富含sEVs部分中miR - 132和miR - 26b的表达增加。血清生化指标正常的肥胖雌性犬组以及与miR - 155表达相关的方面未观察到变化。这些结果表明，患有高甘油三酯血症的肥胖雌性犬可能在sEVs以及与脂质代谢和脂肪生成相关的miRNAs表达方面出现改变。
