银屑病皮损与非皮损的mRNA表达谱

The mRNA Expression Profile of Psoriatic Lesion Distinct from Non-Lesion.

作者信息

Li Xinhua, Xing Jianxiao, Wang Fangdi, Li Juan, Li Junqin, Hou Ruixia, Zhang Kaiming

机构信息

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, 030009, People's Republic of China.

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Taiyuan Central Hospital, Taiyuan, Shanxi Province, 030009, People's Republic of China.

出版信息

Clin Cosmet Investig Dermatol. 2022 Sep 27;15:2035-2043. doi: 10.2147/CCID.S385894. eCollection 2022.

DOI:10.2147/CCID.S385894

PMID:36193053

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9526433/

Abstract

PURPOSE

Psoriasis is a chronic recurring autoimmune skin disease with a complex etiology and chronic progression; however, its molecular mechanisms remain unclear.

PATIENTS AND METHODS

We performed transcriptomic analysis to profile the mRNA expression of psoriatic lesions (PL) and non-lesion (NL) tissues from psoriasis patients along with normal skin from healthy donors. RT-qPCR was used to validate the mRNA expression profiles.

RESULTS

A total of 237 differentially expressed genes were screened and identified by RNA sequencing. GO and KEGG analysis indicated that these DEGs were enriched in the PPAR signaling pathway and intermediate filament cytoskeleton. For PPAR signaling pathway, the expression of five genes, including ADIPOQ, AQP7, PLIN1, FABP4 and LPL, were all significantly decreased in psoriatic lesions compared to normal skin by RT-qPCR. There is a clear difference between psoriatic lesions and non-lesion in the expression of ADIPOQ, AQP7 and LPL. For intermediate filament cytoskeleton, including KRT27, KRT25, KRT71, KRT86 and KRT85 were significantly decreased in the psoriasis lesions, showing agreement with the RNA-seq data.

CONCLUSION

This study revealed a significant difference between the mRNA expression profiles of PL, NL tissue and normal skin.

摘要

目的

银屑病是一种病因复杂、病程慢性复发的自身免疫性皮肤病；然而，其分子机制仍不清楚。

患者和方法

我们进行了转录组分析，以描绘银屑病患者的银屑病皮损（PL）和非皮损（NL）组织以及健康供体正常皮肤的mRNA表达谱。采用RT-qPCR验证mRNA表达谱。

结果

通过RNA测序共筛选并鉴定出237个差异表达基因。GO和KEGG分析表明，这些差异表达基因在PPAR信号通路和中间丝细胞骨架中富集。对于PPAR信号通路，通过RT-qPCR检测，与正常皮肤相比，包括ADIPOQ、AQP7、PLIN1、FABP4和LPL在内的5个基因在银屑病皮损中的表达均显著降低。ADIPOQ、AQP7和LPL在银屑病皮损和非皮损中的表达存在明显差异。对于中间丝细胞骨架，包括KRT27、KRT25、KRT71、KRT86和KRT85在银屑病皮损中显著降低，与RNA-seq数据一致。