Combined Evaluation of mRNA and Protein Expression, Promoter Methylation, and Immune Infiltration of UBE2I in Pan-Digestive System Tumors.

BACKGROUND

Digestive system tumors (DSTs) have high morbidity and mortality worldwide. This study explored the potential value of ubiquitin-conjugating enzyme E2 I () in pan-digestive system tumors (pan-DSTs).

METHODS

Differential expression, tumor stages, and survival outcomes of in pan-DSTs were determined using the GEPIA database. The TIMER database was used to confirm the correlation of expression with pan-DSTs and immune infiltrates. Differential analyses of promoter methylation and protein levels were performed using the UALCAN database. The underlying mechanisms of involvement in pan-DSTs were visualized using interaction networks. The diagnostic value of in pan-DSTs was identified using the Oncomine database.

RESULTS

was differentially and highly expressed in cholangiocarcinoma (CHOL), pancreatic adenocarcinoma (PAAD), colon adenocarcinoma (COAD), rectal adenocarcinoma (READ), liver hepatocellular carcinoma (LIHC), and stomach adenocarcinoma (STAD). According to survival analysis, upregulated was associated with adverse overall and disease-free survival in PAAD and favorable overall survival in READ. expression was partially linked to the purity of immune infiltration in COAD, LIHC, PAAD, READ, and STAD, as indicated by the immune infiltration analysis. Promoter methylation analysis showed differential and high methylation of in PAAD as well as stratified analysis by gender, nodal metastasis, and race. Protein expression analysis in colon cancer revealed that had differential and high expression in tumors as well as stratified analysis by gender, tumor histology, race, and tumor stage. Mechanism explorations demonstrated that in COAD and PAAD, was involved in spliceosomal snRNP complex, Notch signaling pathway, etc. Diagnostic analysis indicated that had consistent diagnostic value for COAD and PAAD.

CONCLUSIONS

Upregulated may be a diagnostic and surveillance predictive signature for PAAD and COAD. The potential significance of immune infiltrates and promoter methylation in PAAD and COAD needs further exploration.