利用[具体内容未给出]生物合成氧化锌纳米颗粒及其在MCF-7和A549细胞系中抗癌特性的演变 。
Biosynthesis of ZnONP Using and Their Evolution of Anticancer Property in MCF-7 and A549 Cell Lines.
作者信息
Balaji Menaka Priya, Govindasamy Rajakumar, Alharbi Naiyf S, Kadaikunnan Shine, Thiruvengadam Muthu, Baskar Venkidasamy, Devi Rajeswari Vijayarangan
机构信息
Department of Biomedical Sciences, School of Bioscience and Technology, VIT, Vellore 632114, Tamil Nadu, India.
Department of Orthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai 600077, Tamil Nadu, India.
出版信息
Nanomaterials (Basel). 2022 Sep 27;12(19):3384. doi: 10.3390/nano12193384.
The ZnO nanoparticle synthesis using the leaf part of was characterized using UV-Vis spectrophotometry, IR, XRD, DLS, FESEM, EDX, TEM, AFM and XPS. The MTT assay was used to examine the cytotoxicity activity against lung epithelial and breast cell lines, and the IC value was determined. The presence of ZnO nanoparticles, which range in size from 200 to 800 nm, was confirmed by the absorption peak at 350 nm. The median particle size was 145.1 nm, and the ζ -the potential was -19.45 mV, showing that ZnONP is stable. Zinc, carbon, and oxygen contribute to the elemental composition of ZnONP, as determined by EDX analysis. MTT assay was used to investigate in vitro cytotoxicity in MCF-7 and A549 cell lines. The cytotoxicity activity IC value was determined to be 30 μg/mL for the A549 cell line and 37 μg/mL for the MCF-7 cell line.
使用 的叶片部分合成的氧化锌纳米颗粒通过紫外可见分光光度法、红外光谱、X射线衍射、动态光散射、场发射扫描电子显微镜、能谱分析、透射电子显微镜、原子力显微镜和X射线光电子能谱进行表征。采用MTT法检测对肺上皮细胞和乳腺癌细胞系的细胞毒性活性，并测定IC值。通过350nm处的吸收峰证实了尺寸范围为200至800nm的氧化锌纳米颗粒的存在。中位粒径为145.1nm，ζ电位为-19.45mV，表明ZnONP是稳定的。通过能谱分析确定，锌、碳和氧构成了ZnONP的元素组成。采用MTT法研究MCF-7和A549细胞系的体外细胞毒性。A549细胞系的细胞毒性活性IC值测定为30μg/mL，MCF-7细胞系为37μg/mL。
Zotero 插件