精油对儿科痰分离株的抗真菌活性：时间杀伤研究。

Antifungal Activity of Essential Oil against : Time-Kill Study on Pediatric Sputum Isolates.

机构信息

Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.

Department of Microbiology, Faculty of Pharmacy, University of Belgrade, 11000 Belgrade, Serbia.

出版信息

Molecules. 2022 Sep 24;27(19):6300. doi: 10.3390/molecules27196300.

DOI:10.3390/molecules27196300

PMID:36234837

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9571381/

Abstract

The aim of our study was to determine the susceptibility of 15 sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of essential oil (LAEO). The commercial LAEO was analyzed using gas chromatography-mass spectrometry. The antifungal activity was evaluated using EUCAST protocol. A killing assay was performed to evaluate kinetics of 2% LAEO within 30 min treatment. LAEO with major constituents' linalool (33.4%) and linalyl acetate (30.5%) effective inhibited grows of . in concentration range 0.5-2%. Fluconazole activity was noted in 67% of the isolates with MICs in range 0.06-1 µg/mL. Surprisingly, 40% of isolates were non-wild-type (non-WT), while MICs for WT ranged between 0.125-0.25 µg/mL. There were no significant differences in the LAEO MICs among fluconazole-resistant and fluconazole-susceptible sputum strains ( = 0.31) and neither among caspofungin non-WT and WT isolates ( = 0.79). The 2% LAEO rapidly achieved 50% growth reduction in all tested strains between 0.2 and 3.5 min. Within 30 min, the same LAEO concentration exhibited a 99.9% reduction in 27% isolates. This study demonstrated that 2% solution of LAEO showed a significant antifungal activity which is equally effective against fluconazole and caspofungin susceptible and less-susceptible strains.

摘要

我们的研究目的是确定 15 株痰液分离株对氟康唑和卡泊芬净的敏感性，以及精油（LAEO）的抗真菌潜力。使用气相色谱-质谱联用技术对商业 LAEO 进行了分析。使用 EUCAST 方案评估了抗真菌活性。进行了杀菌测定，以评估 2%LAEO 在 30 分钟处理内的动力学。含有主要成分芳樟醇（33.4%）和乙酸芳樟酯（30.5%）的 LAEO 在 0.5-2%浓度范围内有效抑制了的生长。在 67%的分离株中观察到氟康唑活性，MIC 范围为 0.06-1 µg/mL。令人惊讶的是，40%的分离株是非野生型（非 WT），而 WT 的 MIC 范围在 0.125-0.25 µg/mL 之间。在氟康唑耐药和敏感的痰液菌株中，LAEO 的 MIC 之间没有显著差异（=0.31），在卡泊芬净非 WT 和 WT 分离株之间也没有显著差异（=0.79）。在所有测试的菌株中，2%的 LAEO 在 0.2 到 3.5 分钟之间迅速达到 50%的生长减少。在 30 分钟内，相同浓度的 LAEO 在 27%的分离株中表现出 99.9%的减少。本研究表明，2%LAEO 溶液显示出显著的抗真菌活性，对氟康唑和卡泊芬净敏感和不太敏感的菌株同样有效。