品因玫瑰精油通过 NF-κB 通路缓解 LPS 诱导的 RAW264.7 细胞炎症：体外整合和网络药理学分析。

Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.

机构信息

Beijing Engineering and Technology Research Center of Food Additives, College of Food and Health, Beijing Technology and Business University, Building No. 8, Fucheng Road 11#, Haidian District, (BTBU), Beijing, 100048, China.

College of Chemical and Materials Engineering, Beijing Technology and Business University, Building No. 1, Fucheng Road 11#, Haidian District, Beijing, 100048, China.

出版信息

BMC Complement Med Ther. 2022 Oct 14;22(1):272. doi: 10.1186/s12906-022-03748-1.

BACKGROUND

Rosa rugosa cv. Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China. The essential oil of Rosa rugosa cv. Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy. PREO exhibits some anti-inflammation, antioxidant and nerve alleviating effects. However, the mechanisms behind these effects are still unclear.

METHODS

The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress. An inflammatory immune cell model was constructed by exposing RAW 264.7 cells to LPS. A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.

RESULTS

PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities. GC‒MS analysis revealed that PREO consists of 57 compounds, mainly monoterpenoids. Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins. By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling. Molecular docking showed that PREO components can interact with different proteins involved in the NF-κB pathway.

CONCLUSION

The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.

背景

重瓣玫瑰（Rosa rugosa cv. Plena）是玫瑰的一个品种，在中国的医药和食品领域已有超过 1300 年的应用历史。重瓣玫瑰精油（PREO）是食品、化妆品和香薰中最常用的添加剂之一。PREO 具有一定的抗炎、抗氧化和舒缓神经作用。然而，其作用机制尚不清楚。

方法

采用 GC-MS 法测定 PREO 的成分。通过网络药理学预测可能的化合物-靶标网络，并分析针对炎症和氧化应激的可能靶标。采用 LPS 刺激 RAW 264.7 细胞构建炎症免疫细胞模型。通过一系列生化测定、RT-PCR 和 Western blot 实验，研究 PREO 的抗炎和抗氧化作用。

结果

PREO 处理可显著（p<0.05）减轻炎症和氧化生物标志物（如 NO、ROS 和 MDA），并保持 SOD 和 CAT 活性。GC-MS 分析表明，PREO 由 57 种化合物组成，主要为单萜类化合物。网络药理学揭示，香茅醇、法呢醇、辛酸乙酯、乙酸香叶酯和丁香酚是与多种炎症途径蛋白相互作用的活性成分。通过 qRT-PCR 和 Western blot 测定可能靶标的基因和蛋白表达，发现 PREO 在 LPS 处理的 RAW 264.7 细胞中的抗炎反应可能与 NF-κB 信号通路的调节有关。分子对接表明，PREO 成分可与 NF-κB 通路中的不同蛋白相互作用。