转录因子 FfMYB15 在金针菇菌丝生长过程中调节纤维素酶基因 FfCEL6B 的表达。
Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis.
机构信息
College of Food Science and Engineering, Shanxi Agricultural University, 1 Mingxian South Road, Taigu, 030801, Shanxi Province, China.
Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
出版信息
Microb Cell Fact. 2022 Oct 17;21(1):216. doi: 10.1186/s12934-022-01932-z.
BACKGROUND
Cellulose degradation can determine mycelial growth rate and affect yield during the growth of Flammulina filiformis. The degradation of cellulose requires the joint action of a variety of cellulases, and some cellulase-related genes have been detected in mushrooms. However, little is known about the transcriptional regulatory mechanisms of cellulose degradation.
RESULTS
In this study, FfMYB15 that may regulate the expression of cellulase gene FfCEL6B in F. filiformis was identified. RNA interference (RNAi) showed that FfCEL6B positively regulated mycelial growth. Gene expression analyses indicated that the expression patterns of FfCEL6B and FfMYB15 in mycelia cultured on the 0.9% cellulose medium for different times were similar with a correlation coefficient of 0.953. Subcellular localization and transcriptional activity analyses implied that FfMYB15 was located in the nucleus and was a transcriptional activator. Electrophoretic mobility shift assay (EMSA) and dual-luciferase assays demonstrated that FfMYB15 could bind and activate FfCEL6B promoter by recognizing MYB cis-acting element.
CONCLUSIONS
This study indicated that FfCEL6B played an active role in mycelial growth of F. filiformis and was regulated by FfMYB15.
背景
纤维素的降解可以决定金针菇菌丝的生长速率，并影响其生长过程中的产量。纤维素的降解需要多种纤维素酶的共同作用，一些与纤维素酶相关的基因已在蘑菇中被检测到。然而，关于纤维素降解的转录调控机制知之甚少。
结果
本研究鉴定了可能在金针菇中调节纤维素酶基因 FfCEL6B 表达的 FfMYB15。RNA 干扰（RNAi）表明 FfCEL6B 正向调节菌丝生长。基因表达分析表明，在不同时间培养于 0.9%纤维素培养基中的菌丝中，FfCEL6B 和 FfMYB15 的表达模式相似，相关系数为 0.953。亚细胞定位和转录活性分析表明，FfMYB15 位于细胞核内，是一种转录激活因子。电泳迁移率变动分析（EMSA）和双荧光素酶报告基因检测实验表明，FfMYB15 可以通过识别 MYB 顺式作用元件来结合并激活 FfCEL6B 启动子。
结论
本研究表明 FfCEL6B 在金针菇菌丝生长中发挥着积极的作用，并受 FfMYB15 调控。
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