草酸钙晶体与大鼠肾内髓集合管细胞的相互作用。

Calcium oxalate crystal interaction with rat renal inner papillary collecting tubule cells.

作者信息

Wiessner J H, Kleinman J G, Blumenthal S S, Garancis J C, Mandel G S, Mandel N S

出版信息

J Urol. 1987 Sep;138(3):640-3. doi: 10.1016/s0022-5347(17)43289-7.

Abstract

Rat renal inner papillary collecting tubule cells (RPCT) have been isolated and maintained in primary culture. The cells have been found to be of only one type and they have maintained the characteristics of RPCT cells. The RPCT cells in culture appear as a monolayer with intermittent clumps of rounded cells. When small calcium oxalate monohydrate crystals (COM) or calcium oxalate dihydrate crystals (COD) are added to the monolayer of RPCT cells, the crystals bind on or about these clumps of rounded-up cells. The use of this system as a model for the study of crystal membrane interactions in crystalluria and urolithiasis is discussed.

摘要

大鼠肾内髓集合管细胞（RPCT）已被分离并进行原代培养。已发现这些细胞仅为一种类型，并且它们保持了RPCT细胞的特性。培养中的RPCT细胞呈单层，有间歇性的圆形细胞团块。当向RPCT细胞单层中加入小的一水合草酸钙晶体（COM）或二水合草酸钙晶体（COD）时，晶体结合在这些聚集的圆形细胞上或其周围。讨论了将该系统用作研究结晶尿症和尿路结石症中晶体 - 膜相互作用模型的用途。