School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.
School of Life Sciences, Research Center for Industries of the Future, Westlake University, Hangzhou, Zhejiang, China.
Science. 2022 Oct 28;378(6618):405-412. doi: 10.1126/science.abm0646. Epub 2022 Oct 27.
To synthesize a chirally inverted ribosome with the goal of building mirror-image biology systems requires the preparation of kilobase-long mirror-image ribosomal RNAs that make up the structural and catalytic core and about two-thirds of the molecular mass of the mirror-image ribosome. Here, we chemically synthesized a 100-kilodalton mirror-image T7 RNA polymerase, which enabled efficient and faithful transcription of the full-length mirror-image 5, 16, and 23 ribosomal RNAs from enzymatically assembled long mirror-image genes. We further exploited the versatile mirror-image T7 transcription system for practical applications such as biostable mirror-image riboswitch sensor, long-term storage of unprotected kilobase-long l-RNA in water, and l-ribozyme-catalyzed l-RNA polymerization to serve as a model system for basic RNA research.
为了合成具有手性反转核糖体的目的，构建镜像生物系统需要制备千碱基长的镜像核糖体 RNA，这些 RNA 构成了结构和催化核心，以及镜像核糖体三分之二的分子质量。在这里，我们通过化学合成了一个 100 千道尔顿的镜像 T7 RNA 聚合酶，它能够有效地转录全长的镜像 5、16 和 23 核糖体 RNA，这些 RNA 来自酶促组装的长镜像基因。我们进一步利用多功能的镜像 T7 转录系统进行了实际应用，例如生物稳定的镜像核糖体开关传感器、在水中对未保护的千碱基长 l-RNA 的长期储存，以及 l-核酶催化的 l-RNA 聚合，作为基本 RNA 研究的模型系统。
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