Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Methods Mol Biol. 2023;2551:633-647. doi: 10.1007/978-1-0716-2597-2_38.
Amyloidogenesis, self-propagation of protein or peptide monomers to amyloid fibrils, has been linked to incurable pathogenesis of neurodegenerative diseases such as Alzheimer's disease and prion diseases. Investigations of amyloid structures and how monomers are transformed through seeding are therefore crucial for developing therapeutics toward these diseases. Here we describe a cross-seeding method to explore the amyloid core in prion fibrils that uses preformed amyloid fibrils as a seed to induce the transformation of other protein or peptide monomers to amyloid fibrils.
淀粉样蛋白生成，即蛋白质或肽单体自行增殖为淀粉样纤维，与无法治愈的神经退行性疾病（如阿尔茨海默病和朊病毒病）的发病机制有关。因此，研究淀粉样蛋白结构以及单体如何通过成核转化对于开发针对这些疾病的治疗方法至关重要。在这里，我们描述了一种跨成核方法来研究朊病毒纤维中的淀粉样核心，该方法使用预先形成的淀粉样纤维作为种子来诱导其他蛋白质或肽单体转化为淀粉样纤维。
