Microfluidic Integrated Circuits Research Laboratory, Bioengineering, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
Bioengineering, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
Biosensors (Basel). 2022 Nov 9;12(11):995. doi: 10.3390/bios12110995.
Laser-induced graphene (LIG) has been applied in many different sensing devices, from mechanical sensors to biochemical sensors. In particular, LIG fabricated on paper (PaperLIG) shows great promise for preparing cheap, flexible, and disposable biosensors. Distinct from the fabrication of LIG on polyimide, a two-step process is used for the fabrication of PaperLIG. In this study, firstly, a highly conductive PaperLIG is fabricated. Further characterization of PaperLIG confirmed that it was suitable for developing biosensors. Subsequently, the PaperLIG was used to construct a biosensor by immobilizing glucose oxidase, aminoferrocene, and Nafion on the surface. The developed glucose biosensor could be operated at a low applied potential (-90 mV) for amperometric measurements. The as-prepared biosensor demonstrated a limit of detection of (50-75 µM) and a linear range from 100 µM to 3 mM. The influence of the concentration of the Nafion casting solution on the performance of the developed biosensor was also investigated. Potential interfering species in saliva did not have a noticeable effect on the detection of glucose. Based on the experimental results, the simple-to-prepare PaperLIG-based saliva glucose biosensor shows great promise for application in future diabetes management.
激光诱导石墨烯（LIG）已应用于许多不同的传感设备，从机械传感器到生化传感器。特别是，在纸上制造的 LIG（PaperLIG）在制备廉价、灵活和一次性生物传感器方面显示出巨大的潜力。与聚酰亚胺上的 LIG 制造不同，PaperLIG 的制造采用两步工艺。在这项研究中，首先制造出一种高导电性的 PaperLIG。对 PaperLIG 的进一步表征证实，它适用于开发生物传感器。随后，通过将葡萄糖氧化酶、氨基二茂铁和 Nafion 固定在表面上，将 PaperLIG 用于构建生物传感器。所开发的葡萄糖生物传感器可以在低应用电位（-90 mV）下进行安培测量。所制备的生物传感器的检测限为（50-75 µM），线性范围为 100 µM 至 3 mM。还研究了 Nafion 浇铸溶液浓度对开发的生物传感器性能的影响。唾液中的潜在干扰物质对葡萄糖的检测没有明显影响。基于实验结果，这种制备简单的基于 PaperLIG 的唾液葡萄糖生物传感器有望在未来的糖尿病管理中得到应用。
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