Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand.
Molecules. 2022 Oct 26;27(21):7287. doi: 10.3390/molecules27217287.
Portable colorimetric hydrogel test kits are newly developed for the on-site detection of nitrite, nitrate, and phosphate in water. Griess-doped hydrogel was prepared at the bottom of a 1.5 mL plastic tube for nitrite detection, a nitrate reduction film based on zinc powder was placed on the inner lid of a second 1.5 mL plastic tube for use in conjunction with the Griess-doped hydrogel for nitrate detection, and a molybdenum blue-based reagent was entrapped within a poly(vinyl alcohol) hydrogel matrix placed at the bottom of a third 1.5 mL plastic tube to detect phosphate. These test kits are usable with on-mobile digital image colorimetry (DIC) for the on-site determination of nutrients with good analytical performance. The detection limits were 0.02, 0.04, and 0.14 mg L−1 for nitrite, nitrate, and phosphate, respectively, with good accuracy (<4.8% relative error) and precision (<1.85% relative standard deviation). These test kits and on-mobile DIC were used for the on-site determination of nutrients in the Pak Bang and Bang Yai canals, the main canals in Phuket, Thailand. The concentrations of nitrite, nitrate, and phosphate were undetectable to 0.60 mg L−1, undetectable to 2.98 mg L−1, and undetectable to 0.52 mg L−1, respectively.
便携式比色水凝胶测试试剂盒是为现场检测水中的亚硝酸盐、硝酸盐和磷酸盐而新开发的。将格里斯掺杂水凝胶制备在 1.5 mL 塑料管的底部，用于检测亚硝酸盐，将基于锌粉的硝酸盐还原膜放置在第二个 1.5 mL 塑料管的内盖上，与格里斯掺杂水凝胶一起用于硝酸盐检测，将基于钼蓝的试剂包埋在聚（乙烯醇）水凝胶基质中，放置在第三个 1.5 mL 塑料管的底部，用于检测磷酸盐。这些测试试剂盒可与移动数字图像比色法（DIC）一起使用，用于现场测定具有良好分析性能的营养物质。亚硝酸盐、硝酸盐和磷酸盐的检测限分别为 0.02、0.04 和 0.14 mg L−1，具有良好的准确性（<4.8%相对误差）和精密度（<1.85%相对标准偏差）。这些测试试剂盒和移动数字图像比色法用于现场测定泰国普吉岛主要运河 Pak Bang 和 Bang Yai 中的营养物质。亚硝酸盐、硝酸盐和磷酸盐的浓度分别为 0.60 mg L−1 以下不可检测、2.98 mg L−1 以下不可检测和 0.52 mg L−1 以下不可检测。
