Hao Zhao, Wang Yanhong, Guo Xiaofang, De Ji
School of Science, Tibet University, Lhasa, China.
Front Plant Sci. 2022 Oct 28;13:1041504. doi: 10.3389/fpls.2022.1041504. eCollection 2022.
Highland barley ( var. (L.) Hook.f., qingke) has unique physical and chemical properties and good potential for industrial applications. As the only crop that can be grown at high altitudes of 4200-4500 m, qingke is well adapted to extreme habitats at high altitudes. In this study, we analysed the seed bacterial community of 58 genotypes of qingke grown in different regions of Tibet, including qingke landraces, modern cultivars, and winter barley varieties, and characterised endophytic bacterial communities in seeds from different sources and the core endo-bacteriome of qingke. This study aim to provide a reference for the application of seed endophytes as biological inoculants for sustainable agricultural production and for considering microbe-plant interactions in breeding strategies. A total of 174 qingke seed samples from five main agricultural regions in Tibet were collected and subjected to investigation of endophytic endo-bacteriome using high-throughput sequencing and bioinformatics approaches. The phyla of endophytic bacteria in qingke seeds from different sources were similar; however, the relative proportions of each phylum were different. Different environmental conditions, growth strategies, and modern breeding processes have significantly changed the community structure of endophytic bacteria in seeds, among which the growth strategy has a greater impact on the diversity of endophytic bacteria in seeds. Seeds from different sources have conserved beneficial core endo-bacteriome. The core endo-bacteriome of qingke seeds dominated by Enterobacteriaceae may maintain qingke growth by promoting plant growth and assisting plants in resisting pests and diseases. This study reveals the core endo-bacteriome of qingke seeds and provides a basis for exploiting the endophytic endo-bacteriome of qingke seeds.
青稞（变种（L.）Hook.f.，藏语称“青稞”）具有独特的理化性质，在工业应用方面具有良好潜力。作为唯一能在海拔4200 - 4500米的高海拔地区种植的作物，青稞非常适应高海拔的极端生境。在本研究中，我们分析了生长于西藏不同地区的58种青稞基因型的种子细菌群落，包括青稞地方品种、现代栽培品种和冬大麦品种，并对不同来源种子中的内生细菌群落以及青稞的核心内生细菌组进行了表征。本研究旨在为种子内生菌作为可持续农业生产的生物接种剂的应用以及在育种策略中考虑微生物 - 植物相互作用提供参考。从西藏五个主要农业地区共收集了174份青稞种子样本，并采用高通量测序和生物信息学方法对内生细菌组进行了调查。不同来源的青稞种子中的内生细菌门类相似；然而，各门类的相对比例不同。不同的环境条件、生长策略和现代育种过程显著改变了种子中内生细菌的群落结构，其中生长策略对种子内生细菌的多样性影响更大。不同来源的种子具有保守的有益核心内生细菌组。以肠杆菌科为主的青稞种子核心内生细菌组可能通过促进植物生长和协助植物抵御病虫害来维持青稞生长。本研究揭示了青稞种子的核心内生细菌组，并为开发青稞种子的内生细菌组提供了依据。
