健康汉族成年人中多态性与工作记忆的关联

Association of polymorphisms with working memory in healthy Han Chinese adults.

作者信息

Ma Hui, Qiu Riyang, Zhang Wenya, Chen Xiaohong, Zhang Liguo, Wang Man

机构信息

Hainan Provincial Institute of Mental Health, Hainan Provincial Anning Hospital, Haikou, Hainan, China.

Department of Precision Therapy, Shenzhen Kangning Hospital, Shenzhen, Guangdong, China.

出版信息

Front Neurosci. 2022 Nov 10;16:989046. doi: 10.3389/fnins.2022.989046. eCollection 2022.

DOI:10.3389/fnins.2022.989046

PMID:36440265

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9685989/

Abstract

AIMS

The dopamine- and cAMP-regulated phosphoprotein (DARPP-32), which is encoded by the gene, plays a converging regulatory role in the central nervous system by mediating the actions of dopamine, serotonin, and glutamate. Previous studies have demonstrated that variations in genes related to the dopamine system influence working memory. The present study thus investigated whether polymorphisms in gene were associated with working memory.

MATERIALS AND METHODS

A sample of 124 healthy Han Chinese were genotyped for three single nucleotide polymorphisms of gene, namely rs12601930C/T, rs879606A/G, and rs3764352A/G, using polymerase chain reaction and restriction fragment length polymorphism analysis. Working memory performance was assessed using the Wisconsin Card Sorting Test (WCST).

RESULTS

Significant differences were observed in the Total Correct (TC), Total Errors (TE), and Conceptual Level Responses (CLR) scores of the WCST among the three rs12601930C/T genotypes ( = 0.044, 0.044, and 0.047, respectively); in TC, TE, Non-Perseverative Errors (NPE), and CLR scores between participants with the CC and (CT + TT) rs12601930C/T polymorphism genotypes ( = 0.032, 0.032, 0.019, and 0.029, respectively); in TC, TE, Perseverative Errors (PE), NPE, and CLR scores between participants with the (CT + CC) and TT rs12601930C/T polymorphism genotypes ( = 0.001, 0.001, 0.011, 0.004, and 0.001, respectively); and in NPE and CLR scores between participants with the GG and (AG + AA) genotypes of the rs3764352A/G polymorphism ( = 0.011 and 0.010). Furthermore, for males only, there were significant differences in TC, TE, PE, NPE, and CLR scores among the rs12601930C/T genotypes ( = 0.020, 0.020, 0.037, 0.029, and 0.014, respectively) and NPE and CLR scores among the rs3764352 genotypes ( = 0.045 and 0.042).

CONCLUSION

gene polymorphisms rs12601930C/T and rs3764352A/G might be associated with working memory assessed by the WCST in healthy Chinese adults, especially among males.

摘要

目的

由该基因编码的多巴胺和环磷酸腺苷调节磷蛋白（DARPP - 32）通过介导多巴胺、血清素和谷氨酸的作用，在中枢神经系统中发挥汇聚调节作用。先前的研究表明，与多巴胺系统相关的基因变异会影响工作记忆。因此，本研究调查了该基因的多态性是否与工作记忆相关。

材料与方法

使用聚合酶链反应和限制性片段长度多态性分析，对124名健康汉族人样本进行该基因的三个单核苷酸多态性（即rs12601930C/T、rs879606A/G和rs3764352A/G）的基因分型。使用威斯康星卡片分类测验（WCST）评估工作记忆表现。

结果

在rs12601930C/T三种基因型的WCST总正确数（TC）、总错误数（TE）和概念水平反应（CLR）得分中观察到显著差异（分别为 = 0.044、0.044和0.047）；在rs12601930C/T多态性基因型为CC和（CT + TT）的参与者之间的TC、TE、非持续性错误（NPE）和CLR得分（分别为 = 0.032、0.032、0.019和0.029）；在rs12601930C/T多态性基因型为（CT + CC）和TT的参与者之间的TC、TE、持续性错误（PE）、NPE和CLR得分（分别为 = 0.001、0.001、0.011、0.004和0.001）；以及在rs3764352A/G多态性基因型为GG和（AG + AA）的参与者之间的NPE和CLR得分（ = 0.011和0.010）。此外，仅对于男性，rs12601930C/T基因型之间的TC、TE、PE、NPE和CLR得分存在显著差异（分别为 = 0.020、0.020、0.037、0.029和0.014），rs3764352基因型之间的NPE和CLR得分存在显著差异（ = 0.045和0.042）。