Lee Keunsub, Wang Kan
Department of Agronomy, Iowa State University, Ames, IA 50011, USA; Crop Bioengineering Center, Iowa State University, Ames, IA 50011, USA.
Department of Agronomy, Iowa State University, Ames, IA 50011, USA; Crop Bioengineering Center, Iowa State University, Ames, IA 50011, USA.
Curr Opin Biotechnol. 2023 Feb;79:102848. doi: 10.1016/j.copbio.2022.102848. Epub 2022 Dec 1.
Recent advances in the genome-editing tools have demonstrated a great potential for accelerating functional genomics and crop trait improvements, but the low efficiency and genotype dependence in plant transformation hinder practical applications of such revolutionary tools. Morphogenic transcription factors (MTFs) such as Baby boom, Wuschel2, GROWTH-REGULATING FACTOR5, GROWTH-REGULATING FACTOR4 and its cofactor GRF-INTERACTING FACTOR1, and Wuschel-homeobox 5 related have been shown to greatly enhance plant transformation efficiency and expand the range of amenable species and genotypes. This review will summarize recent advancements in plant transformation technologies with an emphasis on the strategies developed for genotype-flexible transformation methods utilizing MTFs for both monocots and dicot plant species. We highlight several breakthrough studies that demonstrated a wide range of applicability.
基因组编辑工具的最新进展已显示出在加速功能基因组学和作物性状改良方面的巨大潜力，但植物转化中的低效率和基因型依赖性阻碍了这类革命性工具的实际应用。诸如“婴儿潮”、“Wuschel2”、“生长调控因子5”、“生长调控因子4”及其辅因子“生长调控因子相互作用因子1”以及“Wuschel同源异型盒5相关因子”等形态发生转录因子已被证明能极大提高植物转化效率，并扩大适用物种和基因型的范围。本综述将总结植物转化技术的最新进展，重点介绍为利用形态发生转录因子实现单子叶和双子叶植物基因型灵活转化方法所开发的策略。我们着重介绍了几项证明具有广泛适用性的突破性研究。
