β-榄香烯调节上皮-间充质转化，抑制结直肠癌细胞的侵袭和转移。
β-Elemene regulates epithelial-mesenchymal transformation and inhibits invasion and metastasis of colorectal cancer cells.
机构信息
Department of Proctology, Second Hospital Affiliated Anhui University of Traditional Chinese Medicine, Hefei, P.R. China.
Graduate Department, Anhui University of Chinese Medicine, Hefei, P.R. China.
出版信息
J Complement Integr Med. 2022 Dec 9;20(2):425-430. doi: 10.1515/jcim-2022-0295. eCollection 2023 Jun 1.
OBJECTIVES
To study the inhibitory effect of β-elemene on invasion and metastasis of colorectal cancer cells and its possible mechanism.
METHODS
Human colon cancer HCT116 cells were treated with different concentrations of β-elemene. The proliferation inhibition rate of the cells was detected by MTT assay, cell migration rate was detected by scratched assay, and cell invasion rate was evaluated by Transwell cell invasion assay. The expressions of Vimentin, E-cadherin, N-cadherin, and β-catenin were detected by Western blotting. The mRNA expressions of Vimentin, E-cadherin, N-cadherin, and β-catenin were detected by real-time PCR.
RESULTS
Compared with the control group, the expressions of migration rate, invasion rate, scratch healing rate, N-cadherin, and Vimentin protein of HCT116 cells were decreased after β-elemene treatment, while the expression of E-cadherin protein was increased, and the inhibition rate of cell proliferation was increased (p<0.05).
CONCLUSIONS
β-Elemene may inhibit cell proliferation and invasion and metastasis by inhibiting EMT signaling pathway in human colon cancer cell line HCT116.
目的
研究β-榄香烯对结直肠癌细胞侵袭转移的抑制作用及其可能的机制。
方法
用不同浓度β-榄香烯处理人结肠癌细胞 HCT116，MTT 法检测细胞增殖抑制率，划痕实验检测细胞迁移率，Transwell 细胞侵袭实验评价细胞侵袭率，Western blot 检测 Vimentin、E-cadherin、N-cadherin 和 β-catenin 的表达，实时 PCR 检测 Vimentin、E-cadherin、N-cadherin 和 β-catenin 的 mRNA 表达。
结果
与对照组相比，β-榄香烯处理后 HCT116 细胞的迁移率、侵袭率、划痕愈合率、N-cadherin 和 Vimentin 蛋白表达降低，E-cadherin 蛋白表达增加，细胞增殖抑制率增加（p<0.05）。
结论
β-榄香烯可能通过抑制 EMT 信号通路抑制人结肠癌细胞系 HCT116 的细胞增殖、侵袭和转移。
Zotero 插件