肾去神经对大鼠急性心肌梗死后室性心律失常的影响及机制。
Effects and mechanism of renal denervation on ventricular arrhythmia after acute myocardial infarction in rats.
机构信息
Department of Cardiology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, People's Republic of China.
Shanghai Putuo Central School of Clinical Medicine, Anhui Medical University, Shanghai, 200062, People's Republic of China.
出版信息
BMC Cardiovasc Disord. 2022 Dec 12;22(1):544. doi: 10.1186/s12872-022-02980-4.
BACKGROUND
Renal denervation (RDN) can reduce ventricular arrhythmia after acute myocardial infarction (AMI), but the mechanism is not clear. The purpose of this study is to study its mechanism.
METHODS
Thirty-two Sprague-Dawley rats were divided into four groups: control group, AMI group, RDN-1d + AMI group, RDN-2w + AMI group. The AMI model was established 1 day after RDN in the RDN-1d + AMI group and 2 weeks after RDN in the RDN-2w + AMI group. At the same time, 8 normal rats were subjected to AMI modelling (the AMI group). The control group consisted of 8 rats without RDN intervention or AMI modelling.
RESULTS
The study confirmed that RDN can reduce the occurrence of ventricular tachycardia in AMI rats, reduce renal sympathetic nerve discharge, and inhibit the activity of local sympathetic nerves and cell growth factor (NGF) protein expression in the heart after AMI. In addition, RDN decreased the expression of norepinephrine (NE) and glutamate in the hypothalamus,and NE in cerebrospinal fluid, and increased the expression level of γ aminobutyric acid (GABA) in the hypothalamus after AMI.
CONCLUSION
RDN can effectively reduce the occurrence of ventricular arrhythmia after AMI, and its main mechanism may be via the inhibition of central sympathetic nerve discharge.
背景
肾脏去神经支配（RDN）可减少急性心肌梗死（AMI）后室性心律失常，但机制尚不清楚。本研究旨在探讨其机制。
方法
32 只 Sprague-Dawley 大鼠随机分为 4 组：对照组、AMI 组、RDN-1d+AMI 组、RDN-2w+AMI 组。RDN-1d+AMI 组在 RDN 后 1 天建立 AMI 模型，RDN-2w+AMI 组在 RDN 后 2 周建立 AMI 模型。同时，8 只正常大鼠进行 AMI 建模（AMI 组）。对照组包括 8 只未进行 RDN 干预或 AMI 建模的大鼠。
结果
研究证实，RDN 可减少 AMI 大鼠室性心动过速的发生，降低肾脏交感神经放电，并抑制 AMI 后心脏局部交感神经活性和细胞生长因子（NGF）蛋白表达。此外，RDN 降低了 AMI 后下丘脑去甲肾上腺素（NE）和谷氨酸水平以及脑脊液中 NE 水平，并增加了下丘脑 γ 氨基丁酸（GABA）的表达水平。
结论
RDN 可有效减少 AMI 后室性心律失常的发生，其主要机制可能是通过抑制中枢交感神经放电。
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