Genome-Wide Identification and Expression Analysis of the Gene Family of the Drumstick Tree ( Lam.) Reveals Regulatory Effects on Shoot Regeneration.

Auxin plays a critical role in organogenesis in plants. The classical auxin signaling pathway holds that auxin initiates downstream signal transduction by degrading Aux/IAA transcription repressors that interact with ARF transcription factors. In this study, 23 genes were identified in the drumstick tree genome. All genes were located within five subfamilies based on phylogenetic evolution analysis; the gene characteristics and promoter cis-elements were also analyzed. The protein interaction network between the MoIAAs with MoARFs was complex. The gene family responded positively to NAA treatment, exhibiting different patterns and degrees, notably for , and . The three genes expressed and functioned in the nucleus; only the intact encoding protein of exhibited transcriptional activation activity. The shoot regeneration capacity in the transgenic line was considerably lower than in the wild type. These results establish a foundation for further research on gene function and provide useful information for improved tissue culture efficiency and molecular breeding of .