基于人工核苷酸适配体的场效应晶体管用于肝癌外泌体的超灵敏检测
Artificial Nucleotide Aptamer-Based Field-Effect Transistor for Ultrasensitive Detection of Hepatoma Exosomes.
作者信息
Chen Yiheng, Kong Derong, Qiu Liping, Wu Yungen, Dai Changhao, Luo Shi, Huang Zhipeng, Lin Qiuyuan, Chen Hui, Xie Sitao, Geng Li, Zhao Jun, Tan Weihong, Liu Yunqi, Wei Dacheng
机构信息
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
Institute of Molecular Materials and Devices, Fudan University, Shanghai 200433, China.
出版信息
Anal Chem. 2023 Jan 17;95(2):1446-1453. doi: 10.1021/acs.analchem.2c04433. Epub 2022 Dec 28.
An aptamer-based field-effect transistor (Apta-FET) is a well-developed assay method with high selectivity and sensitivity. Due to the limited information density that natural nucleotide library holds, the Apta-FET faces fundamental restriction in universality to detect various types of analytes. Herein, we demonstrate a type of Apta-FET sensors based on an artificial nucleotide aptamer (AN-Apta-FET). The introduction of an artificial nucleotide increases the diversity of the potential aptamer structure and expands the analyte category of the Apta-FET. The AN-Apta-FET specifically detects hepatoma exosomes, which traditional Apta-FET fails to discriminate from other tumor-derived exosomes, with a limit of detection down to 242 particles mL. The AN-Apta-FET distinguishes serum samples of hepatocellular carcinoma patients within 9 min from those of healthy people, showing the potential as a comprehensive assay tool in future disease diagnosis.
基于适配体的场效应晶体管（Apta-FET）是一种成熟的检测方法，具有高选择性和高灵敏度。由于天然核苷酸文库的信息密度有限，Apta-FET在检测各种类型分析物的通用性上面临根本限制。在此，我们展示了一种基于人工核苷酸适配体的Apta-FET传感器（AN-Apta-FET）。人工核苷酸的引入增加了潜在适配体结构的多样性，并扩大了Apta-FET的分析物类别。AN-Apta-FET能够特异性检测肝癌外泌体，而传统Apta-FET无法将其与其他肿瘤来源的外泌体区分开来，检测限低至242个颗粒/毫升。AN-Apta-FET能够在9分钟内区分肝细胞癌患者和健康人的血清样本，显示出其在未来疾病诊断中作为综合检测工具的潜力。
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