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CRISPR/Cas9 是加速商业棕榈树遗传改良的可行方法吗?前景与局限。

Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits.

作者信息

Khan Faiza Shafique, Goher Farhan, Zhang Dapeng, Shi Peng, Li Zhiying, Htwe Yin Min, Wang Yong

机构信息

Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions/Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan, China.

Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan, China.

出版信息

Front Plant Sci. 2022 Dec 12;13:1042828. doi: 10.3389/fpls.2022.1042828. eCollection 2022.

Abstract

Commercially important palms (oil palm, coconut, and date palm) are widely grown perennial trees with tremendous commercial significance due to food, edible oil, and industrial applications. The mounting pressure on the human population further reinforces palms' importance, as they are essential crops to meet vegetable oil needs around the globe. Various conventional breeding methods are used for the genetic improvement of palms. However, adopting new technologies is crucial to accelerate breeding and satisfy the expanding population's demands. CRISPR/Cas9 is an efficient genome editing tool that can incorporate desired traits into the existing DNA of the plant without losing common traits. Recent progress in genome editing in oil palm, coconut and date palm are preliminarily introduced to potential readers. Furthermore, detailed information on available CRISPR-based genome editing and genetic transformation methods are summarized for researchers. We shed light on the possibilities of genome editing in palm crops, especially on the modification of fatty acid biosynthesis in oil palm. Moreover, the limitations in genome editing, including inadequate target gene screening due to genome complexities and low efficiency of genetic transformation, are also highlighted. The prospects of CRISPR/Cas9-based gene editing in commercial palms to improve sustainable production are also addressed in this review paper.

摘要

具有商业重要性的棕榈树(油棕、椰子树和枣椰树)是广泛种植的多年生树木,因其在食品、食用油和工业应用方面具有巨大的商业意义。人口增长带来的压力进一步凸显了棕榈树的重要性,因为它们是满足全球植物油需求的重要作物。人们采用了各种传统育种方法来改良棕榈树的基因。然而,采用新技术对于加速育种和满足不断增长的人口需求至关重要。CRISPR/Cas9是一种高效的基因组编辑工具,它可以将所需性状整合到植物现有的DNA中,而不会丢失常见性状。本文向潜在读者初步介绍了油棕、椰子树和枣椰树基因组编辑的最新进展。此外,还为研究人员总结了基于CRISPR的可用基因组编辑和遗传转化方法的详细信息。我们阐述了棕榈作物基因组编辑的可能性,特别是油棕中脂肪酸生物合成的修饰。此外,还强调了基因组编辑的局限性,包括由于基因组复杂性导致的目标基因筛选不足以及遗传转化效率低下。本文还探讨了基于CRISPR/Cas9的基因编辑在商业棕榈树中提高可持续产量的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0916/9791139/3221ffe837c1/fpls-13-1042828-g001.jpg

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