Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, 230032, People's Republic of China.
Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, 230032, People's Republic of China.
Int J Nanomedicine. 2022 Dec 27;17:6593-6606. doi: 10.2147/IJN.S389194. eCollection 2022.
Micro-sized sponge particulates have attracted extensive attention because of their potential to overcome the intrinsic limitations of conventional monolithic scaffolds in tissue engineering. Bioactive nanocomposite microsponges are regarded as potential bone substitute materials for bone regeneration.
Based on a combination of microfluidic emulsion with further freezing and in situ thawing, chitosan (CS)-hydroxyapatite (HAP) microsponges were prepared and characterized in terms of their morphology and elemental distribution using a scanning electron microscope equipped with an X-ray detector. The swelling ratio, porosity, degradability, antibacterial activity, and bioactivity were detected and analyzed. The biological functions of the CS-HAP microsponges were examined to assess the adhesion, proliferation, and differentiation of in vitro co-cultured rat bone marrow mesenchymal stem cells (rBMSCs). Furthermore, the CS-HAP microsponges were used as cell-free scaffolds and implanted into calvarial defects in a rat model to evaluate the in vivo osteogenesis.
The CS-HAP microsponges have a porous structure with high porosity (76%), good swelling capacity (1900%), and shape-memory properties. The results of in vitro experiments show that the CS-HAP microsponges achieve good bioactivity and promote osteogenic differentiation of rBMSCs. Furthermore, the CS-HAP microsponges significantly promote bone regeneration in rat calvarial defects.
The bioactive CS-HAP microsponges have the potential to be used as bone substitute materials for bone tissue engineering.
由于其在组织工程中克服传统整体支架固有局限性的潜力，微尺寸海绵颗粒引起了广泛关注。生物活性纳米复合微海绵被认为是用于骨再生的潜在骨替代材料。
基于微流乳液与进一步冷冻和原位解冻的结合，通过配备 X 射线探测器的扫描电子显微镜对壳聚糖 (CS)-羟基磷灰石 (HAP) 微海绵的形态和元素分布进行了表征。检测和分析了溶胀比、孔隙率、降解性、抗菌活性和生物活性。通过体外共培养大鼠骨髓间充质干细胞 (rBMSCs) 的黏附、增殖和分化来评估 CS-HAP 微海绵的生物学功能。此外，将 CS-HAP 微海绵用作无细胞支架，并植入大鼠颅骨缺损模型中以评估体内成骨作用。
CS-HAP 微海绵具有多孔结构，具有高孔隙率（76%）、良好的溶胀能力（1900%）和形状记忆性能。体外实验结果表明，CS-HAP 微海绵具有良好的生物活性，并促进 rBMSCs 的成骨分化。此外，CS-HAP 微海绵可显著促进大鼠颅骨缺损的骨再生。
生物活性 CS-HAP 微海绵具有作为骨组织工程骨替代材料的潜力。
