Huang Haolin, Li Jingsong, Tao Weiyi, Li Shuang
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
Polymers (Basel). 2022 Dec 23;15(1):56. doi: 10.3390/polym15010056.
The characterization and flocculation mechanism of a biopolymer flocculant produced by sp. HL-1, were investigated. The bio-flocculant HL1 was identified as an acidic polysaccharide, mainly composed of glucose, and also contained a small amount of mannose, galacturonic acid and guluronic acid. The flocculating activity of the purified HL1 polysaccharide could be activated by trivalent cations, and its flocculation mechanism was mainly charge neutralization and bridging. The working concentration of fermentation broth HL1 in a kaolin suspension was only 1/10,000 (/), in which the polysaccharide concentration was about 2 mg/L. The bio-flocculant HL1 maintained high efficiency at a wide range of pH (pH 3-10). It also exhibited good flocculating activity at a temperature range of 20-40 °C; it could even tolerate high salinity and kept activity at a mineralization degree of 50,000 mg/L. Therefore, the bio-flocculant HL1 has a good application prospect in the treatment of wastewater over a broad pH range and in high salinity.
对菌株HL-1产生的一种生物聚合物絮凝剂的特性及絮凝机制进行了研究。生物絮凝剂HL1被鉴定为一种酸性多糖，主要由葡萄糖组成，还含有少量甘露糖、半乳糖醛酸和古洛糖醛酸。纯化后的HL1多糖的絮凝活性可被三价阳离子激活，其絮凝机制主要是电荷中和及架桥作用。发酵液HL1在高岭土悬浮液中的有效浓度仅为万分之一（/），其中多糖浓度约为2 mg/L。生物絮凝剂HL1在较宽的pH范围（pH 3 - 10）内保持高效。它在20 - 40 °C的温度范围内也表现出良好的絮凝活性；甚至能够耐受高盐度，在矿化度为50,000 mg/L时仍保持活性。因此，生物絮凝剂HL1在宽pH范围及高盐度废水处理方面具有良好的应用前景。
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