Liu Yang, Nakatsu Yoshimichi, Tanaka Hiromasa, Koga Kazunori, Ishikawa Kenji, Shiratani Masaharu, Hori Masaru
Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Genes Environ. 2023 Jan 13;45(1):3. doi: 10.1186/s41021-023-00260-x.
Non-thermal atmospheric pressure plasma technologies form the core of many scientific advances, including in the electronic, industrial, and biotechnological fields. The use of plasma as a cancer therapy has recently attracted significant attention due to its cancer cell killing activity. Plasma-activated Ringer's lactate solution (PAL) exhibits such activity. In addition to ROS, PAL contains active compounds or species that cause cancer cell death, but the potential mutagenic risks of PAL have not been studied.
PAL has a low pH value and a high concentration of HO. HO was removed from PAL using catalase and catalase-treated PAL with a pH of 5.9 retained a killing effect on HeLa cells whereas this effect was not observed if the PAL was adjusted to pH 7.2. Catalase-treated PAL at pH 5.9 had no significant effect on mutation frequency, the expression of γH2AX, or G2 arrest in HeLa cells.
PAL contains one or more active compounds or species in addition to HO that have a killing effect on HeLa cells. The compound(s) is active at lower pH conditions and apparently exhibits no genotoxicity. This study suggested that identification of the active compound(s) in PAL could lead to the development of novel anticancer drugs for future cancer therapy.
非热大气压等离子体技术构成了许多科学进步的核心，包括在电子、工业和生物技术领域。由于其癌细胞杀伤活性，等离子体作为一种癌症治疗方法最近受到了广泛关注。等离子体激活的乳酸林格氏液（PAL）具有这种活性。除了活性氧（ROS），PAL还含有导致癌细胞死亡的活性化合物或物质，但尚未研究PAL的潜在诱变风险。
PAL具有低pH值和高浓度的羟基自由基（HO）。使用过氧化氢酶从PAL中去除HO，pH为5.9的经过氧化氢酶处理的PAL对HeLa细胞仍保留杀伤作用，而如果将PAL的pH值调至7.2，则未观察到这种效果。pH为5.9的经过氧化氢酶处理的PAL对HeLa细胞的突变频率、γH2AX的表达或G2期阻滞没有显著影响。
除HO外，PAL还含有一种或多种对HeLa细胞具有杀伤作用的活性化合物或物质。该化合物在较低pH条件下具有活性，且显然没有遗传毒性。这项研究表明，鉴定PAL中的活性化合物可能会导致开发用于未来癌症治疗的新型抗癌药物。
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