哺乳动物中DNA N-甲基腺嘌呤修饰的鉴定与定量：对现代分析技术的一项挑战。

Identification and quantification of DNA N-methyladenine modification in mammals: A challenge to modern analytical technologies.

作者信息

Lyu Cong, Wang Hui-Dong, Lai Weiyi, Wang Hailin

机构信息

The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou, 310024, China; University of Chinese Academy of Sciences, Beijing, China.

出版信息

Curr Opin Chem Biol. 2023 Apr;73:102259. doi: 10.1016/j.cbpa.2022.102259. Epub 2023 Jan 16.

DOI:10.1016/j.cbpa.2022.102259

PMID:36652775

Abstract

DNA N-methyladenine modification (6mA) is a predominant epigenetic mark in prokaryotes but rarely present in multicellular metazoa. The analytical technologies have been developed for sensitive detection of 6mA, including ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) and single molecule real-time sequencing (SMRTseq). However, it remains challenging to detect 6mA at global level and/or in the context of sequence in multicellular metazoa (including mammals). This mini-review brings insights into current dilemma and potential solutions for the identification and quantifications of 6mA in mammals.

摘要

DNA N-甲基腺嘌呤修饰（6mA）是原核生物中主要的表观遗传标记，但在多细胞后生动物中很少见。已经开发出用于灵敏检测6mA的分析技术，包括超高效液相色谱-质谱联用（UHPLC-MS/MS）和单分子实时测序（SMRTseq）。然而，在多细胞后生动物（包括哺乳动物）中，在全局水平和/或序列背景下检测6mA仍然具有挑战性。这篇小型综述深入探讨了当前在哺乳动物中鉴定和定量6mA的困境及潜在解决方案。