血小板衍生生长因子A和B信使核糖核酸的长3'非翻译区仅有远缘关系。
The long 3'-untranslated regions of the PDGF-A and -B mRNAs are only distantly related.
作者信息
Hoppe J, Schumacher L, Eichner W, Weich H A
机构信息
Department of Cytogenetics, GBF-Gesellschaft für Biotechnologische Forschung mbH., Braunschweig, FRG.
出版信息
FEBS Lett. 1987 Nov 2;223(2):243-6. doi: 10.1016/0014-5793(87)80297-1.
PMID:3666150
Abstract
A cDNA clone of about 2300 base pairs was prepared from the human osteosarcoma cell line U-2 OS by hybridization with a 22-mer oligonucleotide complementary to the NH2-terminus of PDGF-A. Restriction and sequence analysis showed that this clone contains the entire coding region for PDGF-A and a long 3'-untranslated region which is only distantly related to that in the mRNA of PDGF-B.
摘要
通过与一条与血小板衍生生长因子A（PDGF-A）氨基末端互补的22聚体寡核苷酸杂交，从人骨肉瘤细胞系U-2 OS制备了一个约2300个碱基对的cDNA克隆。限制性酶切和序列分析表明，该克隆包含PDGF-A的完整编码区和一个长的3'非翻译区，该区域与PDGF-B mRNA中的相应区域只有远缘关系。
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