S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000 - Jardim Seminario, Campo Grande-MS, 79117-900, Brazil.
Programa de Pós-Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia) Universidade Federal de Juiz de Fora, Campus Universitário, Rua José Lourenço Kelmer, s/n - São Pedro, Juiz de Fora-MG, 36036-900, Brazil.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac009.
Bacterial resistance is a threat to health worldwide, mainly due to reduced effective treatment. In this context, the search for strategies to control such infections and suppress antimicrobial resistance is necessary. One of the strategies that has been used is combination therapy. In the present work, we investigated the in vitro efficacy of the antimicrobials diminazene aceturate (DA), chloramphenicol (CHL), and streptomycin (STP) alone and in combination against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus clinical isolates. DA was capable of inhibiting all strains with MIC of 25-400 μg mL-1, while STP and CHL showed antibacterial activity with minimum inhibitory concentration (MICs) of ≤3.12-400 μg mL-1. The combination of aceturate with STP showed synergism toward almost all Gram-negative bacteria, with fractional inhibitory concentration index (FICIs) of 0.09-0.37. In addition, for CHL and aceturate, synergisms for Gram-negative and -positive strains were observed. A time-kill assay against E. coli revealed that the aceturate and STP combination can inhibit bacterial growth in a shorter time when compared with single antibiotics. In addition, antimicrobials did not show hemolytic activity even at the highest concentrations used. Therefore, the antimicrobial combinations presented in this work showed important results, demonstrating that combined therapy can be used as an alternative strategy for pathogen control.
细菌耐药性是全球范围内的健康威胁，主要是由于有效治疗的减少。在这种情况下，有必要寻找控制这些感染和抑制抗菌药物耐药性的策略。已经使用的策略之一是联合治疗。在本工作中，我们研究了单独使用和联合使用抗菌药物乙酰氨基苯胂酸（DA）、氯霉素（CHL）和链霉素（STP）对大肠杆菌、肺炎克雷伯菌和金黄色葡萄球菌临床分离株的体外疗效。DA 能够抑制所有菌株，其 MIC 为 25-400μg mL-1，而 STP 和 CHL 表现出抗菌活性，最小抑菌浓度（MICs）为 ≤3.12-400μg mL-1。乙酰氨基苯胂酸与 STP 的联合使用对几乎所有革兰氏阴性菌均表现出协同作用，部分抑菌浓度指数（FICIs）为 0.09-0.37。此外，对于 CHL 和乙酰氨基苯胂酸，对革兰氏阴性和阳性菌株均观察到协同作用。针对大肠杆菌的时间杀伤试验表明，与单独使用抗生素相比，乙酰氨基苯胂酸和 STP 的联合使用可以在更短的时间内抑制细菌生长。此外，即使在使用的最高浓度下，抗生素也没有表现出溶血活性。因此，本工作中呈现的抗菌组合表现出重要的结果，表明联合治疗可以用作控制病原体的替代策略。
Zotero 插件