用于真核生物RNA敲低和成像且无毒性的CRISPR-Csm - Suppr

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity.

出版信息

Nat Biotechnol. 2023 Sep;41(9):1204-1205. doi: 10.1038/s41587-023-01665-3.

DOI:10.1038/s41587-023-01665-3

PMID:36693989

Abstract

摘要

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本文引用的文献

CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells.

Nucleic Acids Res. 2022 Jun 24;50(11):e65. doi: 10.1093/nar/gkac159.

Type III CRISPR-Cas Systems: Deciphering the Most Complex Prokaryotic Immune System.

Biochemistry (Mosc). 2021 Oct;86(10):1301-1314. doi: 10.1134/S0006297921100114.

RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.

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相似文献

CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity.

Nat Biotechnol. 2023 Sep;41(9):1204-1205. doi: 10.1038/s41587-023-01665-3.

Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.

Mol Cell. 2019 Jan 17;73(2):264-277.e5. doi: 10.1016/j.molcel.2018.11.007. Epub 2018 Nov 29.

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Science. 2016 Feb 26;351(6276):920-1. doi: 10.1126/science.aaf2851.

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Nat Rev Genet. 2016 Apr;17(4):192-3. doi: 10.1038/nrg.2016.31. Epub 2016 Mar 7.

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Nat Methods. 2014 Apr;11(4):365. doi: 10.1038/nmeth.2912.

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Nat Methods. 2014 Nov;11(11):1090. doi: 10.1038/nmeth.3164.

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引用本文的文献

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本文引用的文献

CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells.

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