绵羊外周血单个核细胞对细粒棘球绦虫微小RNA-71过表达的反应分析
Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression.
作者信息
Li Yating, Yan Lujun, Ci Duojie, Li Rui, Li Wanjing, Xia Tianqi, Shi Hengzhi, Ayaz Mazhar, Zheng Yadong, Wang Pu
机构信息
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China.
NHC Key Laboratory of Echinococcosis Prevention and Control, Tibet Center for Disease Control and Prevention, Lhasa 850000, Tibet Autonomous Region, China.
出版信息
Mol Biochem Parasitol. 2023 Jun;254:111556. doi: 10.1016/j.molbiopara.2023.111556. Epub 2023 Feb 2.
Cyst echinococcosis, caused by Echinococcus granulosus, remains a zoonotic disease posing a great threat to public health and meat production industry. Sheep infected with E. granulosus show relatively high abundance of egr-miR-71 in the sera, but its role is unknown. Using bioinformatics and cell migration and Transwell assays, we comparatively analyzed the proteomes and cell invasion of sheep PBMCs in response to egr-miR-71 overexpression. The results showed that the egr-miR-71 induced a total of 157 proteins being differentially expressed and mainly involved in immune responses. In sheep PBMCs, egr-miRNA-71 overexpression induced significant downregulation of macrophage migration inhibitory factor (MIF) and accordingly promoted cell migration and invasion compared with the control. The results will provide a clue for further investigation of a role of circulating egr-miR-71 in immune responses during E. granulosus infection.
由细粒棘球绦虫引起的囊型棘球蚴病仍然是一种人畜共患病，对公众健康和肉类生产行业构成巨大威胁。感染细粒棘球绦虫的绵羊血清中egr-miR-71丰度相对较高，但其作用尚不清楚。我们利用生物信息学、细胞迁移和Transwell实验，比较分析了绵羊外周血单个核细胞（PBMCs）在egr-miR-71过表达情况下的蛋白质组和细胞侵袭情况。结果表明，egr-miR-71共诱导157种蛋白质差异表达，主要参与免疫反应。在绵羊PBMCs中，与对照组相比，egr-miRNA-71过表达导致巨噬细胞迁移抑制因子（MIF）显著下调，从而促进细胞迁移和侵袭。这些结果将为进一步研究循环egr-miR-71在细粒棘球绦虫感染期间免疫反应中的作用提供线索。
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