两种红毛丹在成熟过程中的代谢组学分析及潜在的味觉生物标志物。
Metabolic Profiling and Potential Taste Biomarkers of Two Rambutans during Maturation.
机构信息
Key Laboratory of Tropical Fruit and Vegetable Cold-Chain of Hainan Province, Institute of Agro-Products of Processing and Design, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572025, China.
出版信息
Molecules. 2023 Feb 1;28(3):1390. doi: 10.3390/molecules28031390.
The metabolite-caused taste variation during rambutan maturation is unknown due to a lack of systematic investigation of all components. In this study, three growing stages, including unripe (S1), half-ripe (S2), and full-ripe (S3) BY2 and BY7 rambutans were compared and profiled by UPLC-MS/MS-based widely targeted metabolomics analysis. We demonstrated that the sugar-acid ratios of two rambutans were greatly improved between the S2 and S3 stages. A total of 821 metabolites were identified, including 232, 205, 204, and 12 differential metabolites (DMs) in BY2-S1 vs. BY2-S2, BY2-S2 vs. BY2-S3, BY7-S1 vs. BY7-S2, and BY7-S2 vs. BY7-S3, respectively. A correlation analysis showed that gamma-aminobutyric acid (GABA) could be the sugar-acid ratio biomarker of BY2 rambutan. Methionine (Met), alanine (Ala), and S-methyl-L-cysteine (SMC) could be total amino acid biomarkers of BY2 and BY7 rambutans. In addition, UPLC-MS/MS-based quantitative verification of the above biomarkers exhibited the same variations as metabolomics analysis. This study not only provides useful nutritive information on rambutans but also valuable metabolic data for rambutan breeding strategies.
由于缺乏对所有成分的系统研究，因此尚不清楚罗望子在成熟过程中代谢物引起的味道变化。在这项研究中，比较并通过基于 UPLC-MS/MS 的广泛靶向代谢组学分析对三个生长阶段（未成熟（S1），半成熟（S2）和完全成熟（S3）BY2 和 BY7 罗望子进行了分析。我们证明，在 S2 和 S3 阶段之间，两种罗望子的糖酸比有了很大提高。共鉴定出 821 种代谢产物，包括 BY2-S1 与 BY2-S2，BY2-S2 与 BY2-S3，BY7-S1 与 BY7-S2 以及 BY7-S2 与 BY7-S3 之间的 232、205、204 和 12 种差异代谢产物（DM）。相关性分析表明，γ-氨基丁酸（GABA）可能是 BY2 罗望子糖酸比的生物标志物。甲硫氨酸（Met），丙氨酸（Ala）和 S-甲基-L-半胱氨酸（SMC）可能是 BY2 和 BY7 罗望子总氨基酸的生物标志物。此外，基于 UPLC-MS/MS 的上述生物标志物的定量验证与代谢组学分析表现出相同的变化。这项研究不仅为罗望子提供了有用的营养信息，而且为罗望子的育种策略提供了有价值的代谢数据。
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