使用 CRISPR/Cas9 技术从 H9 细胞中生成 TRPM8 基因敲除 hESC 系（WAe009-A-A）。
Generation of a TRPM8 knockout hESC line (WAe009-A-A) derived from H9 using CRISPR/Cas9.
机构信息
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Key Laboratory of Hereditary Cardiovascular Diseases, Zhengzhou 450052, China.
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Key Laboratory of Hereditary Cardiovascular Diseases, Zhengzhou 450052, China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China.
出版信息
Stem Cell Res. 2023 Mar;67:103040. doi: 10.1016/j.scr.2023.103040. Epub 2023 Feb 6.
The transient receptor potential cation channel subfamily M member 8 (TRPM8) is a kind of non-selective cation channel which controls Ca homeostasis. Mutations in TRPM8 were related to dry eye diseases (DED). Here we constructed a TRPM8 knockout cell line WAe009-A-A from the original embryonic stem cell line H9 using CRISPR/Cas9 technology, which maybe helpful for exploring the pathogenesis of DED. WAe009-A-A cells possess stem cell morphology and pluripotency as well as normal karyotype, and have the ability of differentiating into three germ layers in vitro.
瞬时受体电位阳离子通道亚家族 M 成员 8（TRPM8）是一种非选择性阳离子通道，可控制 Ca 稳态。TRPM8 的突变与干眼病（DED）有关。在这里，我们使用 CRISPR/Cas9 技术从原始胚胎干细胞系 H9 中构建了 TRPM8 敲除细胞系 WAe009-A-A，这可能有助于探索 DED 的发病机制。WAe009-A-A 细胞具有干细胞形态和多能性以及正常核型，并具有体外分化为三个胚层的能力。
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