Zhang Rui, Li Chang, Yu Mengjun, Huang Xiaoyun, Zhang Mengqi, Liu Shanshan, Pan Shanshan, Xue Weizhen, Wang Congyan, Mao Chunyan, Zhang He, Fan Guangyi
BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China.
Department of Biology, Hong Kong Baptist University, Hong Kong, China.
GigaByte. 2021 Apr 1;2021:gigabyte17. doi: 10.46471/gigabyte.17. eCollection 2021.
The humpback puffer, , is a poisonous freshwater pufferfish species mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). The humpback puffer has many interesting biological features, such as inactivity, tetrodotoxin production and body expansion. Here, we report the first chromosome-level genome assembly of the humpback puffer. The genome size is 362 Mb, with a contig N50 value of ∼1.78 Mb and a scaffold N50 value of ∼15.8 Mb. Based on this genome assembly, ∼61.5 Mb (18.11%) repeat sequences were identified, 19,925 genes were annotated, and the function of 90.01% of these genes could be predicted. Finally, a phylogenetic tree of ten teleost fish species was constructed. This analysis suggests that the humpback puffer and share a common ancestor 18.1 million years ago (MYA), and diverged from 45.8 MYA. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance.
驼背河豚是一种有毒的淡水河豚物种，主要分布在东南亚（泰国、老挝、马来西亚和印度尼西亚）。驼背河豚具有许多有趣的生物学特征，如不活动、产生河豚毒素和身体膨胀。在此，我们报告了驼背河豚的首个染色体水平的基因组组装。基因组大小为362 Mb，重叠群N50值约为1.78 Mb，支架N50值约为15.8 Mb。基于该基因组组装，鉴定出约61.5 Mb（18.11%）的重复序列，注释了19,925个基因，其中90.01%的基因功能可被预测。最后，构建了十种硬骨鱼物种的系统发育树。该分析表明，驼背河豚与[某物种]在1810万年前（百万年前）有共同祖先，并在458万年前与[某物种]分化。驼背河豚基因组将是阐明河豚毒素合成和耐受性可能机制的宝贵基因组资源。
