School of Ophthalmology & Optometry, Wenzhou Medical University, Wenzhou 325035, China.
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China.
Biosensors (Basel). 2023 Jan 20;13(2):164. doi: 10.3390/bios13020164.
Sialic acid (SA) is a well-known component of glycoproteins, which have applications in various functional processes on the cell's surface. The colorimetric is a simpler and more convenient method for measuring SA due to its low-cost apparatus and visual signal changes. This work focused on the unpredictable interparticle crosslinking aggregation of the functionalized gold nanoparticles (AuNPs) in complex media. We proposed a balance of the Derjaguin-Landau-Verwey-Overbeek (DLVO)-type aggregation and molecule-based interaction method to solve this problem. Here, we report a novel colorimetric assay for the determination of SA using 4-mercaptophenyl boronic acid (4-MPBA) as an analyte's recognition molecule, and negative charge PEG was used to repulsive the interparticle crosslinking. The proposed sensing platform shows a linear relationship between the ratio of the absorbance intensity (A/A) and concentration of SA from 0.05 to 8 mM (R = 0.997) and a detection limit of 48 μM was observed. The novel gold-based colorimetric sensor is easy to fabricate, reproducible in its test performance and has been successfully applied for the detection of SA in biological and healthcare product samples.
唾液酸（SA）是糖蛋白的一个众所周知的组成部分，在细胞表面的各种功能过程中有应用。比色法由于其低成本的仪器和可视化信号变化，是一种更简单、更方便的测量 SA 的方法。本工作主要关注功能化金纳米粒子（AuNPs）在复杂介质中不可预测的颗粒间交联聚集。我们提出了一种德热那广义-朗道-弗韦尔-奥弗贝克（DLVO）型聚集和基于分子相互作用的平衡方法来解决这个问题。在这里，我们报告了一种使用 4-巯基苯硼酸（4-MPBA）作为分析物识别分子的新型比色测定法来测定 SA，并用带负电荷的 PEG 来排斥颗粒间的交联。所提出的传感平台在 0.05 至 8 mM 的范围内显示出吸光度强度比（A/A）与 SA 浓度之间的线性关系（R = 0.997），并且观察到检测限为 48 μM。这种新型的基于金的比色传感器易于制造，在测试性能方面具有可重复性，并已成功应用于生物和医疗产品样品中 SA 的检测。
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