孔源性视网膜脱离患者玻璃体内血管内皮生长因子的浓度

Vitreal Concentrations of Vascular Endothelial Growth Factor in Patients with Rhegmatogenous Retinal Detachment.

作者信息

Hasanpour Hossein, Kenney Maria Cristina, Kuppermann Baruch D, Esfahani Mohammad Riazi, Kanavi Mozhgan Rezaei, Singh Mithalesh Kumar, Soheilian Masoud

机构信息

Gavin Herbert Eye Institute, Department of Ophthalmology, University of California Irvine, Irvine, CA 92697, USA.

Mitochondrial Research, Gavin Herbert Eye Institute Ophthalmology, University of California Irvine, Irvine, CA 92697, USA.

出版信息

J Clin Med. 2023 Feb 5;12(4):1259. doi: 10.3390/jcm12041259.

DOI:10.3390/jcm12041259

PMID:36835796

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9961416/

Abstract

The purpose of this study is to evaluate the concentration of vascular endothelial growth factor (VEGF) in the vitreous humor of patients with primary rhegmatogenous retinal detachment (RRD). This is a prospective case control study. Eighteen patients with primary RRD without proliferative vitreoretinopathy C (PVR C) were enrolled as cases, and twenty-two non-diabetic retinopathy patients who were candidates for complete pars plana vitrectomy due to Macular Hole or Epiretinal Membrane were included as the control group. Undiluted vitreal samples were collected during the initiation of Pars Plana Vitrectomy (PPV) prior to any infusion into the posterior cavity. Vitreous samples were also collected from 21 fresh cadaveric globes. The vitreous concentration of VEGF was measured by enzyme-linked immunosorbent assay (ELISA) technique and compared between these two groups. The vitreal concentration of VEGF was 0.643 ± 0.088 ng/mL in the RRD group. Measured concentrations of VEGF in controls were 0.043 ± 0.104 ng/mL, and in cadaveric eyes they were 0.033 ± 0.058 ng/mL. The mean VEGF concentration in the RRD group was statistically higher than in the control group ( < 0.0001) and cadaveric eyes ( < 0.0001). Our study shows that vitreal VEGF concentrations significantly increase in patients with RRD.

摘要

本研究的目的是评估原发性孔源性视网膜脱离（RRD）患者玻璃体液中血管内皮生长因子（VEGF）的浓度。这是一项前瞻性病例对照研究。纳入18例无增殖性玻璃体视网膜病变C级（PVR C级）的原发性RRD患者作为病例组，纳入22例因黄斑裂孔或视网膜前膜而拟行完全玻璃体切割术的非糖尿病性视网膜病变患者作为对照组。在进行玻璃体切割术（PPV）开始时，在未向眼后腔注入任何物质之前收集未稀释的玻璃体液样本。还从21个新鲜尸体眼球中收集玻璃体液样本。采用酶联免疫吸附测定（ELISA）技术测量VEGF的玻璃体液浓度，并在两组之间进行比较。RRD组VEGF的玻璃体液浓度为0.643±0.088 ng/mL。对照组VEGF的测量浓度为0.043±0.104 ng/mL，尸体眼球中的浓度为0.033±0.058 ng/mL。RRD组VEGF的平均浓度在统计学上高于对照组（<0.0001）和尸体眼球（<0.0001）。我们的研究表明，RRD患者玻璃体液中VEGF浓度显著升高。