揭示小麦镁转运蛋白家族基因在非生物胁迫响应中的作用。

Uncovering the role of wheat magnesium transporter family genes in abiotic responses.

作者信息

Tang Yanhong, Yang Xiaoyue, Li Han, Shuai Yating, Chen Wang, Ma Dongfang, Lü Zhichuang

机构信息

MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province)/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, China.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

出版信息

Front Plant Sci. 2023 Feb 9;14:1078299. doi: 10.3389/fpls.2023.1078299. eCollection 2023.

BACKGROUND

The CorA / MGT / MRS2 family proteins are an important group of magnesium transporter proteins that maintain magnesium ion homeostasis in plant cells. However, little is known about the MGT functions in wheat.

METHODS

The known MGT sequences were used as queries to BlastP against wheat genome IWGSC RefSeq v2.1 assembly (E-value <10-5). Chromosome localization information for each gene was obtained from the GFF3 file of the wheat genome data (IWGSCv2.1).The sequence of 1500 bp upstream of the genes was extracted from the wheat genome data. The cis-elements were analyzed using PlantCARE online tool.

RESULT

A total of 24 genes were identified on 18 chromosomes of wheat. After functional domain analysis, only , , and had GMN mutations to AMN, while all the other genes had conserved GMN tripeptide motifs. Expression profiling showed that the genes were differentially expressed under different stresses and at different growth and development stages. The expression levels of and were significantly up-regulated in cold damage. In addition, qRT-PCR results also confirmed that these genes are involved in the wheat abiotic stress responses.

CONCLUSION

In conclusion, The results of our research provide a theoretical basis for further research on the function of gene family in wheat.

背景

CorA / MGT / MRS2家族蛋白是一类重要的镁转运蛋白，可维持植物细胞中的镁离子稳态。然而，关于小麦中MGT的功能知之甚少。

方法

以已知的MGT序列作为查询序列，对小麦基因组IWGSC RefSeq v2.1组装进行BlastP比对（E值<10-5）。每个基因的染色体定位信息从小麦基因组数据的GFF3文件（IWGSCv2.1）中获得。从小麦基因组数据中提取基因上游1500 bp的序列。使用PlantCARE在线工具分析顺式元件。