应用绿色技术从禽畜废水中生长的生物质中提取清洁安全的生物活性化合物。
Application of Green Technology to Extract Clean and Safe Bioactive Compounds from Biomass Grown in Poultry Wastewater.
机构信息
Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, 21000 Novi Sad, Serbia.
Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, 21000 Novi Sad, Serbia.
出版信息
Molecules. 2023 Mar 6;28(5):2397. doi: 10.3390/molecules28052397.
Microalgae are capable of assimilating nutrients from wastewater (WW), producing clean water and biomass rich in bioactive compounds that need to be recovered from inside the microalgal cell. This work investigated subcritical water (SW) extraction to collect high-value compounds from the microalga after treating poultry WW. The treatment efficiency was evaluated in terms of total Kjeldahl nitrogen (TKN), phosphate, chemical oxygen demand (COD) and metals. was able to remove 77% TKN, 50% phosphate, 84% COD, and metals (48-89%) within legislation values. SW extraction was performed at 170 °C and 30 bar for 10 min. SW allowed the extraction of total phenols (1.073 mg GAE/mL extract) and total flavonoids (0.111 mg CAT/mL extract) with high antioxidant activity (IC value, 7.18 µg/mL). The microalga was shown to be a source of organic compounds of commercial value (e.g., squalene). Finally, the SW conditions allowed the removal of pathogens and metals in the extracts and residues to values in accordance with legislation, assuring their safety for feed or agriculture applications.
微藻能够从废水中（WW）吸收养分，生产清洁水和富含生物活性化合物的生物质，这些生物活性化合物需要从微藻细胞内回收。本研究调查了亚临界水（SW）提取技术，以从处理过的家禽 WW 中的微藻中收集高价值化合物。该处理的效率是根据总凯氏氮（TKN）、磷酸盐、化学需氧量（COD）和金属来评估的。该处理能够在法规值内去除 77%的 TKN、50%的磷酸盐、84%的 COD 和 48-89%的金属。SW 提取在 170°C 和 30 巴下进行 10 分钟。SW 允许提取总酚（1.073mgGAE/mL 提取物）和总类黄酮（0.111mgCAT/mL 提取物），具有高抗氧化活性（IC 值为 7.18μg/mL）。微藻被证明是具有商业价值的有机化合物（如角鲨烯）的来源。最后，SW 条件允许提取物和残留物中的病原体和金属去除到符合法规的水平，确保它们在饲料或农业应用中的安全性。
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