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新西兰特有的两种濒危桃金娘科属植物——[具体属名1]和[具体属名2]的种子储存生理学

Seed Storage Physiology of and , Two Threatened Myrtaceae Genera Endemic to New Zealand.

作者信息

van der Walt Karin, Nadarajan Jayanthi

机构信息

Ōtari Native Botanic Garden, Wellington City Council, 150 Wilton Road, Wellington 6012, New Zealand.

School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand.

出版信息

Plants (Basel). 2023 Feb 27;12(5):1067. doi: 10.3390/plants12051067.

Abstract

There is no published information on the seed germination or seed storage physiology of , , and This lack of information is hampering conservation efforts of these critically endangered species. This study investigated the seed morphology, seed germination requirements, and long-term seed storage methods for all three species. The impact of desiccation, desiccation and freezing, as well as desiccation plus storage at 5 °C, -18 °C, and -196 °C on seed viability (germination) and seedling vigour was assessed. Fatty acid profiles were compared between and . Variability in storage behaviour between the three species was investigated through differential scanning calorimetry (DSC) by comparing thermal properties of lipids. seed were desiccation-tolerant and viability was retained when desiccated seed was stored for 24 months at 5 °C. seed was both desiccation- and freezing-sensitive, while was desiccation-sensitive. DSC analysis revealed that lipid crystallisation in occurred between -18 °C and -49 °C and between -23 °C and -52 °C in and . It is postulated that the metastable lipid phase, which coincides with the conventional seed banking temperature (i.e., storing seeds at -20 ± 4 °C and 15 ± 3% RH), could cause the seeds to age more rapidly through lipid peroxidation. Seeds of , and are best stored outside of their lipid metastable temperature ranges.

摘要

关于[物种名称1]、[物种名称2]和[物种名称3]的种子萌发或种子储存生理学,尚无公开信息。这一信息缺失阻碍了对这些极度濒危物种的保护工作。本研究调查了这三个物种的种子形态、种子萌发要求以及长期种子储存方法。评估了干燥处理、干燥与冷冻处理,以及在5°C、-18°C和-196°C下干燥加储存对种子活力(萌发)和幼苗活力的影响。比较了[物种名称1]和[物种名称2]之间的脂肪酸谱。通过差示扫描量热法(DSC)比较脂质的热性质,研究了这三个物种在储存行为上的差异。[物种名称1]种子耐干燥,干燥种子在5°C下储存24个月后仍保持活力。[物种名称2]种子对干燥和冷冻均敏感,而[物种名称3]对干燥敏感。DSC分析表明,[物种名称1]中的脂质结晶发生在-18°C至-49°C之间,[物种名称2]和[物种名称3]中的脂质结晶发生在-23°C至-52°C之间。据推测,与传统种子库温度(即种子在-20±4°C和15±3%相对湿度下储存)一致的亚稳脂质相可能会通过脂质过氧化作用使种子更快老化。[物种名称1]、[物种名称2]和[物种名称3]的种子最好在其脂质亚稳温度范围之外储存。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d945/10005796/3d5e58c38d85/plants-12-01067-g001.jpg

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