利用 sp. TTMP20 从甘蔗糖蜜中生产四甲基吡嗪。
Production of Tetramethylpyrazine from Cane Molasses by sp. TTMP20.
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, Guangxi Normal University, Guilin 541006, China.
出版信息
Molecules. 2023 Mar 14;28(6):2640. doi: 10.3390/molecules28062640.
2,3,5,6-Tetramethylpyrazine (TTMP) is an active ingredient of Franch. It can be used in medicine and food fields. In this study, sp. TTMP20 was applied to produce TTMP using cane molasses as a carbon source. After pretreatment with phosphoric acid, 170 mL/L treated molasses, combined with 10 g/L yeast powder, 30 g/L tryptone and 30 g/L (NH)HPO were used for fermentation. After 36 h, TTMP output reached the highest value of 208.8 mg/L. The yield of TTMP using phosphoric acid-treated molasses as carbon source was 145.59% higher than control. Under the sulfuric acid treatment process of molasses (150 g), the maximum yield of TTMP was 895.13 mg/L, which was 183.18% higher than that of untreated molasses (316.1 mg/L). This study demonstrated that molasses is a high-quality and inexpensive carbon source for the manufacture of TTMP, laying the groundwork for the future industrial production of TTMP.
2,3,5,6-四甲基吡嗪（TTMP）是川芎嗪的有效成分之一，可应用于医药和食品领域。本研究以甘蔗糖蜜为碳源，利用 sp. TTMP20 生产 TTMP。经磷酸预处理后，采用 170 mL/L 处理过的甘蔗糖蜜，添加 10 g/L 酵母粉、30 g/L 胰蛋白胨和 30 g/L（NH4）2HPO4 进行发酵。发酵 36 h 后，TTMP 产量达到 208.8 mg/L 的最高值。与对照相比，磷酸处理糖蜜作为碳源的 TTMP 产量提高了 145.59%。在甘蔗糖蜜（150 g）的硫酸处理过程中，TTMP 的最大产量为 895.13 mg/L，比未经处理的甘蔗糖蜜（316.1 mg/L）提高了 183.18%。本研究表明，甘蔗糖蜜是制造 TTMP 的高质量、低成本碳源，为 TTMP 的未来工业化生产奠定了基础。
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