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用于增强骨再生的粘土微粒：体外研究。

Clay microparticles for the enhancement of bone regeneration: in vitro studies.

机构信息

Civil, Environmental Engineering Department, King Fahd University of Petroleum, Minerals (KFUPM), Dhahran, Saudi Arabia.

School of Medicine, Royal College of Surgeons in Ireland (RCSI) Medical University, Busaiteen, Bahrain.

出版信息

Histochem Cell Biol. 2023 Jul;160(1):39-49. doi: 10.1007/s00418-023-02189-2. Epub 2023 Apr 8.

DOI:10.1007/s00418-023-02189-2
PMID:37029830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10082439/
Abstract

Humans develop osteoporosis as they age, a disease characterized by the slow and consistent reduction in bone mass and the subsequent risk of fractures. Due to aging, the mesenchymal stem cells within the bone marrow niche, show a shift in differentiation from osteogenesis to adipogenesis. The challenge of osteoporosis treatment is being met with advances in nanotechnology and tissue engineering. In this study , we evaluated the potential of palygorskite clay mineral microparticles for the promotion of the osteogenic differentiation in human mesenchymal stem cells (hMSCs) in vitro. Alkaline phosphatase (ALP) activity and Alizarin red staining showed that osteogenic differentiation of hMSCs is enhanced in the presence of palygorskite clay. Although, gene expression analysis did not reveal upregulation of several osteogenic markers in the presence of the clay microparticles, another interaction mechanism resulted in the enhanced osteogenic differentiation of hMSCs. The charged surfaces of the palygorskite clay particles interact with the stem cells using their high adhesion characteristics, leading to complete bridging, adherence, and enveloping of the stem cells' cadherins and integrins with their environment. This restoration of the adhesion among the stem cells and their environment most probably promotes/restores the osteogenic differentiation of hMSCs. Therefore, palygorskite clay microparticles are a promising candidate for further in vivo studies on bone regeneration.

摘要

随着年龄的增长，人类会患上骨质疏松症，这种疾病的特征是骨量缓慢而持续地减少，随后骨折的风险增加。由于衰老，骨髓龛中的间充质干细胞分化从成骨向成脂发生转变。骨质疏松症治疗的挑战正迎接着纳米技术和组织工程的进步。在这项研究中，我们评估了凹凸棒石粘土矿物微颗粒在体外促进人骨髓间充质干细胞（hMSCs）成骨分化的潜力。碱性磷酸酶（ALP）活性和茜素红染色表明，凹凸棒石粘土的存在增强了 hMSCs 的成骨分化。尽管基因表达分析显示在粘土微颗粒存在的情况下，几种成骨标志物没有上调，但另一种相互作用机制导致 hMSCs 的成骨分化增强。凹凸棒石粘土颗粒的带电表面通过其高粘附特性与干细胞相互作用，导致干细胞的钙黏蛋白和整合素与周围环境完全桥接、粘附和包裹。这种干细胞与其环境之间的粘附的恢复很可能促进/恢复 hMSCs 的成骨分化。因此，凹凸棒石粘土微颗粒是进一步进行骨再生体内研究的有前途的候选材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/8c806db6556d/418_2023_2189_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/f38ff433ee6c/418_2023_2189_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/881e6596e833/418_2023_2189_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/52714a8ded33/418_2023_2189_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/ea74a0f66ce3/418_2023_2189_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/76df42e9c532/418_2023_2189_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/c1dd08796fde/418_2023_2189_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/8c806db6556d/418_2023_2189_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/f38ff433ee6c/418_2023_2189_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/881e6596e833/418_2023_2189_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/52714a8ded33/418_2023_2189_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/ea74a0f66ce3/418_2023_2189_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/76df42e9c532/418_2023_2189_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/c1dd08796fde/418_2023_2189_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb47/10082439/8c806db6556d/418_2023_2189_Fig7_HTML.jpg

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本文引用的文献

1
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Nanoscale Res Lett. 2020 Aug 28;15(1):173. doi: 10.1186/s11671-020-03403-z.
2
Use of bone turnover markers in postmenopausal osteoporosis.绝经后骨质疏松症中骨转换标志物的应用。
Lancet Diabetes Endocrinol. 2017 Nov;5(11):908-923. doi: 10.1016/S2213-8587(17)30184-5. Epub 2017 Jul 7.
3
Energy Metabolism of the Osteoblast: Implications for Osteoporosis.成骨细胞的能量代谢：对骨质疏松症的影响
Endocr Rev. 2017 Jun 1;38(3):255-266. doi: 10.1210/er.2017-00064.
4
Use of nano-sized clay crystallites to restore adhesion among tumor and aging stem cells - a molecular simulations approach.使用纳米级粘土微晶来恢复肿瘤细胞与衰老干细胞之间的黏附——一种分子模拟方法。
Am J Stem Cells. 2016 Nov 30;5(4):107-115. eCollection 2016.
5
The hallmarks of aging.衰老的特征。
Cell. 2013 Jun 6;153(6):1194-217. doi: 10.1016/j.cell.2013.05.039.
6
Adhesion in the stem cell niche: biological roles and regulation.干细胞龛中的黏附作用：生物学作用和调控。
Development. 2013 Jan 15;140(2):255-65. doi: 10.1242/dev.083139.
7
Rescuing replication and osteogenesis of aged mesenchymal stem cells by exposure to a young extracellular matrix.通过暴露于年轻细胞外基质来拯救衰老间充质干细胞的复制和成骨作用。
FASEB J. 2011 May;25(5):1474-85. doi: 10.1096/fj.10-161497. Epub 2011 Jan 19.
8
Integrins.整合素。
Cell Tissue Res. 2010 Jan;339(1):269-80. doi: 10.1007/s00441-009-0834-6. Epub 2009 Aug 20.
9
Impaired differentiation potential of human trabecular bone mesenchymal stromal cells from elderly patients.老年患者人小梁骨间充质基质细胞分化潜能受损。
Cytotherapy. 2009;11(5):584-94. doi: 10.1080/14653240903079385.
10
Regenerative effects of transplanted mesenchymal stem cells in fracture healing.移植间充质干细胞在骨折愈合中的再生作用。
Stem Cells. 2009 Aug;27(8):1887-98. doi: 10.1002/stem.103.