一项基于人群的抗线粒体 M2 抗体特征研究及其与抗线粒体抗体的一致性。

A population-based characterization study of  anti-mitochondrial M2 antibodies and its consistency with anti-mitochondrial antibodies.

机构信息

Department of Laboratory Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

出版信息

Lab Med. 2023 Nov 2;54(6):618-625. doi: 10.1093/labmed/lmad018.

DOI:10.1093/labmed/lmad018

PMID:37040652

Abstract

OBJECTIVE

This study aims to estimate the prevalence of anti-mitochondrial antibody subtype M2 (AMA-M2) and assess its consistency with AMA in a general population.

METHODS

A total of 8954 volunteers were included to screen AMA-M2 using enzyme-linked immunosorbent assay. Sera with AMA-M2 >50 RU/mL were further tested for AMA using an indirect immunofluorescence assay.

RESULTS

The population frequency of AMA-M2 positivity was 9.67%, of which 48.04% were males and 51.96% were females. The AMA-M2 positivity in males had a peak and valley value of 7.81% and 16.88% in those aged 40 to 49 and ≥70 years, respectively, whereas it showed a balanced age distribution in females. Transferrin and immunoglobulin M were the risk factors for AMA-M2 positivity and exercise was the only protective factor. Of 155 cases with AMA-M2 >50 RU/mL, 25 cases were AMA-positive, with a female-to-male ratio of 5.25:1. Only 2 people, with very high AMA-M2 of 760 and >800 RU/mL, met the diagnostic criteria of primary biliary cholangitis (PBC), making the prevalence of PBC 223.36 per million in southern China.

CONCLUSION

We found that AMA-M2 has a low coincidence rate with AMA in the general population. A new decision-making point for AMA-M2 is needed to improve consistency with AMA and diagnostic accuracy.

摘要

目的

本研究旨在估计抗线粒体抗体亚型 M2（AMA-M2）的流行率，并评估其在一般人群中与 AMA 的一致性。

方法

共纳入 8954 名志愿者，采用酶联免疫吸附试验筛查 AMA-M2。AMA-M2>50 RU/mL 的血清进一步采用间接免疫荧光法检测 AMA。

结果

人群 AMA-M2 阳性率为 9.67%，其中男性占 48.04%，女性占 51.96%。男性 AMA-M2 阳性率在 40 至 49 岁和≥70 岁人群中分别出现峰值和谷值，分别为 7.81%和 16.88%，而女性 AMA-M2 阳性率呈均衡的年龄分布。转铁蛋白和免疫球蛋白 M 是 AMA-M2 阳性的危险因素，运动是唯一的保护因素。在 155 例 AMA-M2>50 RU/mL 的患者中，有 25 例 AMA 阳性，男女比例为 5.25:1。仅有 2 人 AMA-M2 非常高，分别为 760 和>800 RU/mL，符合原发性胆汁性胆管炎（PBC）的诊断标准，使得中国南方 PBC 的患病率为 223.36/百万。