Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
Institute of Food Sciences, CNR-ISA, Via Roma, 64, 83100 Avellino, Italy.
Int J Mol Sci. 2023 Mar 23;24(7):6073. doi: 10.3390/ijms24076073.
Several researchers have demonstrated the health and pharmacological properties of carvacrol and -cymene, monoterpenes of aromatic plants. This study investigated these compounds' possible anti-cholinesterase, anti-α-amylase, and neuroprotective effects. We evaluated the anti-acetylcholinesterase and anti-α-amylase activities at different concentrations of the compounds. The maximum non-toxic dose of carvacrol and -cymene against SH-SY5Y neuroblastoma cells was determined using an MTT assay. The neuroprotective effects of the compounds were evaluated on HO-induced stress in SH-SY5Y cells, studying the expression of caspase-3 using Western blotting assays. Carvacrol showed inhibitory activities against acetylcholinesterase (IC50 = 3.8 µg/mL) and butyrylcholinesterase (IC = 32.7 µg/mL). Instead, the anti-α-amylase activity of carvacrol resulted in an IC value of 171.2 μg/mL After a pre-treatment with the maximum non-toxic dose of carvacrol and -cymene, the expression of caspase-3 was reduced compared to cells treated with HO alone. Carvacrol and -cymene showed in vitro anti-enzymatic properties, and may act as neuroprotective agents against oxidative stress. Further studies are necessary to elucidate their possible use as coadjutants in preventing and treating AD in diabetic patients.
几位研究人员已经证明了香芹酚和 - 侧柏烯（芳香植物的单萜）的健康和药理学特性。本研究调查了这些化合物可能的抗乙酰胆碱酯酶、抗 α-淀粉酶和神经保护作用。我们评估了这些化合物在不同浓度下的抗乙酰胆碱酯酶和抗 α-淀粉酶活性。使用 MTT 测定法确定香芹酚和 - 侧柏烯对 SH-SY5Y 神经母细胞瘤细胞的最大无毒剂量。通过 Western blot 分析研究 caspase-3 的表达，评估化合物对 HO 诱导的 SH-SY5Y 细胞应激的神经保护作用。香芹酚对乙酰胆碱酯酶（IC50 = 3.8 µg/mL）和丁酰胆碱酯酶（IC = 32.7 µg/mL）表现出抑制活性。相反，香芹酚的抗 α-淀粉酶活性导致 IC 值为 171.2 µg/mL。在用香芹酚和 - 侧柏烯的最大无毒剂量预处理后，与单独用 HO 处理的细胞相比，caspase-3 的表达减少。香芹酚和 - 侧柏烯在体外显示出抗酶特性，并且可能作为抗氧化应激的神经保护剂。需要进一步的研究来阐明它们作为辅助剂在预防和治疗糖尿病患者 AD 中的可能用途。
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