Ningxia University, Yinchuan, China.
China Agricultural University, Beijing, China.
PeerJ. 2023 Apr 12;11:e15093. doi: 10.7717/peerj.15093. eCollection 2023.
Skeletal muscle is not only an important tissue involved in exercise and metabolism, but also an important part of livestock and poultry meat products. Its growth and development determines the output and quality of meat to a certain extent, and has an important impact on the economic benefits of animal husbandry. Skeletal muscle development is a complex regulatory network process, and its molecular mechanism needs to be further studied.
We used a weighted co-expression network (WGCNA) and single gene set enrichment analysis (GSEA) to study the RNA-seq data set of bovine tissue differential expression analysis, and the core genes and functional enrichment pathways closely related to muscle tissue development were screened. Finally, the accuracy of the analysis results was verified by tissue expression profile detection and bovine skeletal muscle satellite cell differentiation model (BSMSCs).
In this study, , , , and were identified as marker genes in muscle tissue, which are mainly involved in glycolysis/gluconeogenesis, AMPK pathway and insulin pathway. The assay results showed that these five genes were highly expressed in muscle tissue and positively correlated with the differentiation of bovine BSMSCs.
In this study, several muscle tissue characteristic genes were excavated, which may play an important role in muscle development and provide new insights for bovine molecular genetic breeding.
骨骼肌不仅是参与运动和代谢的重要组织，也是畜肉禽肉产品的重要组成部分。它的生长和发育在一定程度上决定了肉的产量和质量，对畜牧业的经济效益有重要影响。骨骼肌的发育是一个复杂的调控网络过程，其分子机制有待进一步研究。
我们使用加权共表达网络（WGCNA）和单基因集富集分析（GSEA）对牛组织差异表达分析的 RNA-seq 数据集进行研究，筛选与肌肉组织发育密切相关的核心基因和功能富集途径。最后，通过组织表达谱检测和牛骨骼肌卫星细胞分化模型（BSMSCs）验证分析结果的准确性。
本研究鉴定出了 、 、 、 和 作为肌肉组织的标记基因，它们主要参与糖酵解/糖异生、AMPK 途径和胰岛素途径。检测结果表明，这五个基因在肌肉组织中高表达，与牛 BSMSCs 的分化呈正相关。
本研究挖掘出了一些肌肉组织特征基因，它们可能在肌肉发育中发挥重要作用，为牛的分子遗传育种提供了新的见解。
