分泌型 DKK3 对肝癌患者循环 CD56 自然杀伤细胞的影响。
Effect of secretory DKK3 on circulating CD56 natural killer cells in patients with liver cancer.
机构信息
Biological Anthropology Institute, College of Basic Medical Science, Jinzhou Medical University, Jinzhou, Liaoning, P.R. China.
Department of Community Nursing, College of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, P.R. China.
出版信息
Int J Biol Markers. 2023 Jun;38(2):99-104. doi: 10.1177/03936155231169796. Epub 2023 Apr 18.
BACKGROUND
Liver cancer seriously threatens human health. Natural killer (NK) cells are an important part of the innate immune system and have strong anti-tumor ability. Immunotherapy based on NK cells has become a hot topic in the treatment of liver cancer.
METHODS
In this study, we checked the serum DKK3 (sDKK3) and circulating CD56 NK cells using ELISA and flow cytometry, respectively, in the blood of liver cancer patients. The effect on recombinant human DKK3 (rhDKK3) on CD56 NK cells was analyzed in vitro.
RESULTS
We found low levels of sDKK3 in liver cancer patients and a negative correlation between sDKK3 and circulating CD56 NK cells. In addition, we found that DKK3 induced the differentiation and improved the cytotoxicity of CD56 NK cells for the first time. It could be used as an agonist for NK cell-based immunotherapy.
CONCLUSIONS
Improving the clinical efficacy of NK cells through DKK3 will become a new strategy for cancer immunotherapy.
背景
肝癌严重威胁人类健康。自然杀伤 (NK) 细胞是固有免疫系统的重要组成部分，具有强大的抗肿瘤能力。基于 NK 细胞的免疫疗法已成为肝癌治疗的热点。
方法
本研究采用酶联免疫吸附试验（ELISA）和流式细胞术分别检测肝癌患者血清 DKK3（sDKK3）和循环 CD56 NK 细胞，并在体外分析重组人 DKK3（rhDKK3）对 CD56 NK 细胞的影响。
结果
我们发现肝癌患者的 sDKK3 水平较低，sDKK3 与循环 CD56 NK 细胞呈负相关。此外，我们首次发现 DKK3 诱导 CD56 NK 细胞分化并提高其细胞毒性作用，可作为 NK 细胞为基础的免疫治疗的激动剂。
结论
通过 DKK3 提高 NK 细胞的临床疗效将成为癌症免疫治疗的新策略。
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