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多突触末梢是 CA1 海马结构中放射层连接的特征。

Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens.

作者信息

Mark Rigby, Federico W Grillo, Benjamin Compans, Guilherme Neves, Julia Gallinaro, Sophie Nashashibi, Sally Horton, Pedro M Pereira Machado, Maria Alejandra Carbajal, Gema Vizcay-Barrena, Florian Levet, Jean-Baptiste Sibarita, Angus Kirkland, Roland A Fleck, Claudia Clopath, Juan Burrone

机构信息

MRC Centre for Neurodevelopmental Disorders, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK.

MRC Centre for Neurodevelopmental Disorders, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; The Rosalind Franklin Institute, Harwell Campus, Didcot OX11 0FA, UK.

出版信息

Cell Rep. 2023 May 30;42(5):112397. doi: 10.1016/j.celrep.2023.112397. Epub 2023 Apr 18.

DOI:10.1016/j.celrep.2023.112397
PMID:37074915
原文链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC10695768/
Abstract

Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a single postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to the CA1 region of the hippocampus. Roughly half of all excitatory synapses in the stratum oriens involved multi-synaptic boutons (MSBs), where a single presynaptic bouton containing multiple active zones contacted many postsynaptic spines (from 2 to 7) on the basal dendrites of different cells. The fraction of MSBs increased during development (from postnatal day 22 [P22] to P100) and decreased with distance from the soma. Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared with neighboring SSBs, features that were confirmed by super-resolution light microscopy. Computer simulations suggest that these properties favor synchronous activity in CA1 networks.

摘要

兴奋性突触通常被描述为单个突触小泡 (SSB)，其中一个突触前小泡接触单个突触后棘突。使用连续切片块面扫描电子显微镜，我们发现这种突触的教科书定义不完全适用于海马 CA1 区。大约一半的兴奋性突触在层状或区域涉及多突触小泡 (MSB)，其中一个包含多个活性区的单个突触前小泡接触到不同细胞的基底树突上的许多突触后棘突 (从 2 到 7)。MSB 的分数在发育过程中增加（从出生后第 22 天 [P22] 到 P100），并随距离细胞体的增加而减少。奇怪的是，与相邻的 SSB 相比，MSB 内的突触特性（如活性区 (AZ) 或突触后密度 (PSD) 大小）的变化较小，这些特性通过超分辨率荧光显微镜得到了证实。计算机模拟表明，这些特性有利于 CA1 网络中的同步活动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/ad985f30f8a7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/e01f66aae0fd/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/86554ab9b8fc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/2d6caa538a48/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/6833239d5193/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/ad985f30f8a7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/e01f66aae0fd/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/86554ab9b8fc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/2d6caa538a48/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/6833239d5193/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a05/10695768/ad985f30f8a7/gr4.jpg

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